MGI phenotypes (allele matching)
FVB.129P2(B6)-Mfge8Gt(KST227)Byg/Orl
| Status | Available to order |
| EMMA ID | EM:04424 |
| Citation information | RRID:IMSR_EM:04424 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB.129P2(B6)-Mfge8Gt(KST227)Byg/Orl |
| Alternative name | MFGE8/TMbetageo FVB |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Mfge8Gt(KST227)Byg |
| Gene/Transgene symbol | Mfge8 |
Information from provider
| Provider | clotilde thery |
| Provider affiliation | INSERM U932, Institut Curie |
| Genetic information | Insertion of the genetrap vector pGT1.8TM (containing beta-galactosidase and neomycin resistance genes) downstream the 7th intron of Mfge8 gene. |
| Phenotypic information | Functionally knock-out for Mfge8. Expression of beta-galactosidase and neomycin resistance under the control of the Mfge8 promoter. |
| Breeding history | Original mice obtained from 129/Ola gene-trapped ES cells were backcrossed 8 times to C57BL/6 background. Resulting colony was used to generate this mouse line, by performing 10 backcrosses to FVB/N background. No functional studies have been performed on any homozygous animals in the FVB pure genetic background: reported data are only inferred from the phenotypes in C57BL/6 mice. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous FVB males, wild-type FVB females |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal spleen morphology / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- impaired macrophage phagocytosis / MGI
- abnormal lactation / MGI
- reduced male fertility / MGI
- decreased litter size / MGI
- abnormal kidney physiology / MGI
- abnormal epididymis morphology / MGI
- abnormal cell adhesion / MGI
- abnormal physiological neovascularization / MGI
- abnormal milk composition / MGI
- impaired acrosome reaction / MGI
- increased anti-double stranded DNA antibody level / MGI
- epididymis epithelium degeneration / MGI
- increased spleen weight / MGI
- immune system phenotype / MGI
- abnormal mammary gland growth during lactation / MGI
- abnormal involution of the mammary gland / MGI
- abnormal spleen B cell follicle morphology / MGI
- increased spleen white pulp amount / MGI
- abnormal mammary gland duct morphology / MGI
- spermatic granuloma / MGI
- mastitis / MGI
- hypolactation / MGI
Literature references
- Capturing genes encoding membrane and secreted proteins important for mouse development.;Skarnes W C, Moss J E, Hurtley S M, Beddington R S, ;1995;Proceedings of the National Academy of Sciences of the United States of America;92;6592-6; 7604039
- Lactadherin promotes VEGF-dependent neovascularization.;Silvestre Jean-Sébastien, Théry Clotilde, Hamard Ghislaine, Boddaert Jacques, Aguilar Barbara, Delcayre Alain, Houbron Christophe, Tamarat Radia, Blanc-Brude Olivier, Heeneman Sylvia, Clergue Michel, Duriez Micheline, Merval Régine, Lévy Bernard, Tedgui Alain, Amigorena Sebastian, Mallat Ziad, ;2005;Nature medicine;11;499-506; 15834428
- Mfge8 is critical for mammary gland remodeling during involution.;Atabai Kamran, Fernandez Rafael, Huang Xiaozhu, Ueki Iris, Kline Ahnika, Li Yong, Sadatmansoori Sepid, Smith-Steinhart Christine, Zhu Weimin, Pytela Robert, Werb Zena, Sheppard Dean, ;2005;Molecular biology of the cell;16;5528-37; 16195353