B6.Cg-Tg(Afp-lacZ)1Scnch/Orl
| Status | Available to order |
| EMMA ID | EM:04533 |
| Citation information | RRID:IMSR_EM:04533 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Afp-lacZ)1Scnch/Orl |
| Alternative name | AFP/ßGal |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Afp-lacZ)1Scnch |
| Gene/Transgene symbol | Tg(Afp-lacZ)1Scnch |
Information from provider
| Provider | Sophie Conchon |
| Provider affiliation | Biothérapies hépatiques, INSERM U948 |
| Genetic information | A transgenic construct containing a gene encoding ß-galactosidase under the control of the rat alpha-fetoprotein promoter, was injected into B6D2 donor eggs. Founder lines were subsequently established and bred to C57BL/6 mice. |
| Phenotypic information | Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express ß-galactosidase (ßGal) under the control of the alpha-fetoprotein promoter. ßGal expression is detected in fetal hepatocytes and is repressed shortly after birth. This mutant mouse strain may be useful in developmental studies as well as in studies on immunity against self-antigen. |
| Breeding history | Backcrossed on C57BL/6 mice (8-10 generations). Strain currently inbred. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous males, wild-type C57BL/6J females |
Literature references
- A transgenic mouse with beta-Galactosidase as a fetal liver self-antigen for immunotherapy studies.;Cany Jeannette, Avril Audrey, Pichard Virginie, Aubert Dominique, Ferry Nicolas, Conchon Sophie, ;2007;Journal of hepatology;47;396-403; 17462783
- DNA/amphiphilic block copolymer nanospheres promote low-dose DNA vaccination.;McIlroy Dorian, Barteau Benoît, Cany Jeannette, Richard Peggy, Gourden Clothilde, Conchon Sophie, Pitard Bruno, ;2009;Molecular therapy : the journal of the American Society of Gene Therapy;17;1473-81; 19417740