B6.Cg-Cd247^tm1Mal/Orl

Status	Available to order
EMMA ID	EM:00046
Citation information	RRID:IMSR_EM:00046 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Cd247^tm1Mal/Orl
Alternative name	CD3 z/eta°
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cd247^tm1Mal
Gene/Transgene symbol	Cd247

Information from provider

Provider	M Malissen
Provider affiliation	U136, CIML
Phenotypic information	Mutant mice deficient in CD3 antigen zeta/eta (Cd247) gene, obtained by mutation of the gene by homologous recombination (insertion of the neomycin-resistance gene into the second exon) in ES cells from a C57BL/6 line. The homozygous mutant animals have a profound reduction in the surface levels of T cell receptor (TCR) complexes into thymocyte populations. The products of the CD3-zeta/eta gene appear to be needed for the survival of CD4+CD8+ thymocytes.
Breeding history	Bruce 4 ES cells. Chimaeras crossed with C57BL/6L; one cross with B10.BR then H2^b selection and intercross.
References	T cell development in mice lacking the CD3-zeta/eta gene.;Malissen M, Gillet A, Rocha B, Trucy J, Vivier E, Boyer C, Köntgen F, Brun N, Mazza G, Spanopoulou E, ;1993;The EMBO journal;12;4347-55; 8223444

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	heterozygous C57BL/6 males

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

T-B+ severe combined immunodeficiency due to CD3delta/CD3epsilon/CD3zeta / Orphanet_169160

MGI phenotypes (allele matching)

abnormal T cell differentiation / MGI
abnormal double-negative T cell morphology / MGI
abnormal thymocyte activation / MGI
decreased double-positive T cell number / MGI
abnormal T cell morphology / MGI
decreased thymocyte number / MGI
increased double-negative T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
decreased single-positive T cell number / MGI
abnormal intraepithelial T cell morphology / MGI

MGI phenotypes (gene matching)

enlarged spleen / MGI
enlarged lymph nodes / MGI
small thymus / MGI
decreased thymocyte number / MGI
abnormal superior colliculus morphology / MGI
reduced long term depression / MGI
thymus hypoplasia / MGI
abnormal long term depression / MGI
absent long term depression / MGI
abnormal T cell differentiation / MGI
abnormal brain morphology / MGI
no abnormal phenotype detected / MGI
abnormal CNS synaptic transmission / MGI
abnormal double-negative T cell morphology / MGI
abnormal T-helper 1 cell morphology / MGI
abnormal T cell physiology / MGI
abnormal excitatory postsynaptic potential / MGI
enhanced long term potentiation / MGI
increased natural killer cell mediated cytotoxicity / MGI
abnormal thymocyte activation / MGI
abnormal lateral geniculate nucleus morphology / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
abnormal regulatory T cell physiology / MGI
increased regulatory T cell number / MGI
abnormal NK cell morphology / MGI
increased double-negative T cell number / MGI
decreased double-positive T cell number / MGI
abnormal T cell proliferation / MGI
decreased T cell proliferation / MGI
immune system phenotype / MGI
abnormal CD4-positive, alpha-beta T cell physiology / MGI
abnormal T-helper 1 physiology / MGI
abnormal NK T cell morphology / MGI
abnormal T cell morphology / MGI
decreased NK T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
abnormal single-positive T cell number / MGI
decreased single-positive T cell number / MGI
abnormal T-helper 1 cell differentiation / MGI
absent gamma-delta intraepithelial T cell / MGI
abnormal gamma-delta T cell differentiation / MGI
abnormal alpha-beta intraepithelial T cell morphology / MGI
enlarged lateral ventricles / MGI
increased interferon-gamma secretion / MGI
abnormal intraepithelial T cell morphology / MGI
abnormal bone marrow cell physiology / MGI
decreased splenocyte proliferation / MGI
decreased thymocyte apoptosis / MGI
abnormal NK cell physiology / MGI
postnatal lethality, complete penetrance / MGI
small thymus medulla / MGI
enlarged thymus cortex / MGI

Literature references

T cell development in mice lacking the CD3-zeta/eta gene.;Malissen M, Gillet A, Rocha B, Trucy J, Vivier E, Boyer C, Köntgen F, Brun N, Mazza G, Spanopoulou E, ;1993;The EMBO journal;12;4347-55; 8223444
Impaired spatial memory in mice lacking CD3ζ is associated with altered NMDA and AMPA receptors signaling independent of T-cell deficiency.;Louveau Antoine, Angibaud Julie, Haspot Fabienne, Opazo Maria Cecilia, Thinard Reynald, Thepenier Virginie, Baudouin Stéphane J, Lescaudron Laurent, Hulin Philippe, Riedel Claudia A, Boudin Hélène, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;18672-85; 24259588

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.Cg-Cd247tm1Mal/Orl