IMPC phenotypes (gene matching)
B6.129P2-Gpx4tm1Marc/Ieg
| Status | Available to order |
| EMMA ID | EM:04603 |
| Citation information | RRID:IMSR_EM:04603 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Gpx4tm1Marc/Ieg |
| Alternative name | nuclear GPx4 KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gpx4tm1Marc |
| Gene/Transgene symbol | Gpx4 |
Information from provider
| Provider | Marcus Conrad |
| Provider affiliation | Institut of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum Muenchen |
| Genetic information | The EGFP gene was inserted into the first intron of Gpx4 such that it replaced most of the coding region of the alternative first exon. A translational stop cassette was inserted downstream of EGFP. In situ hybridization confirmed the absence of expression in mutant testis. Western blot failed to detect the nuclear protein form in mutants. |
| Phenotypic information | Male mice are fully fertile with a delay in chromatin condensation of sperm nuclei in the epididymis. |
| Breeding history | Backcrossed to C57BL/6J for 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6N males, wild-type C57BL/6N females |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Spondylometaphyseal dysplasia, Sedaghatian type / Orphanet_93317
MGI phenotypes (allele matching)
- reproductive system phenotype / MGI
MGI phenotypes (gene matching)
- impaired fertilization / MGI
- abnormal apoptosis / MGI
- increased mortality induced by gamma-irradiation / MGI
- abnormal germ layer development / MGI
- failure to gastrulate / MGI
- decreased embryo size / MGI
- reduced male fertility / MGI
- male infertility / MGI
- decreased litter size / MGI
- abnormal embryonic tissue morphology / MGI
- abnormal extraembryonic tissue morphology / MGI
- no abnormal phenotype detected / MGI
- abnormal sperm motility / MGI
- oxidative stress / MGI
- abnormal sperm physiology / MGI
- cellular phenotype / MGI
- reproductive system phenotype / MGI
- abnormal mitochondrial physiology / MGI
- abnormal sperm head morphology / MGI
- kinked sperm flagellum / MGI
- decreased fertilization frequency / MGI
- empty decidua capsularis / MGI
- abnormal sperm midpiece morphology / MGI
- abnormal sperm mitochondrial sheath morphology / MGI
- abnormal sperm principal piece morphology / MGI
- embryonic lethality, complete penetrance / MGI
- embryonic lethality between implantation and placentation, complete penetrance / MGI
- embryonic lethality between implantation and somite formation, complete penetrance / MGI
- embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
Literature references
- The nuclear form of phospholipid hydroperoxide glutathione peroxidase is a protein thiol peroxidase contributing to sperm chromatin stability.;Conrad M, Moreno S G, Sinowatz F, Ursini F, Kölle S, Roveri A, Brielmeier M, Wurst W, Maiorino M, Bornkamm G W, ;2005;Molecular and cellular biology;25;7637-44; 16107710