IMPC phenotypes (gene matching)
B6.129(Cg)-P2ry14tm1.1Gac/Orl
| Status | Available to order |
| EMMA ID | EM:04645 |
| Citation information | RRID:IMSR_EM:04645 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129(Cg)-P2ry14tm1.1Gac/Orl |
| Alternative name | KOP2Y14-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | P2ry14tm1.1Gac |
| Gene/Transgene symbol | P2ry14 |
Information from provider
| Provider | Christian Gachet |
| Provider affiliation | UMR Inserm UdS 949, EFS-Alsace |
| Genetic information | The KO construct P2Y14 (P2ry14) -/- was obtained by deletion of the coding sequence. The line has been obtained by deletion of the P2Y14 CDS by 2 steps: 1) homologous recombination using a construct where loxP sites were inserted from part to part of the CDS. 2) deletion of the CDS by crossing the mice with mice expressing the Cre recombinase under the control of ubiquitous promoter, and selection of mice able to transmit the deletion to its progeny. PCR reaction performed with total DNA and CDS internal primer does not allow any sequence to be amplified from these mice, demonstrating total deletion of the CDS and absence of random integration of the vector used for the construct. |
| Phenotypic information | No phenotype revealed so far. |
| Breeding history | Backcrossed 8 generations with C57BL/6J mice. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
MGI phenotypes (gene matching)
- abnormal muscle physiology / MGI
- decreased airway responsiveness / MGI
- increased heart rate / MGI
- decreased circulating insulin level / MGI
- no phenotypic analysis / MGI
- decreased insulin secretion / MGI
- increased mean systemic arterial blood pressure / MGI
- increased spleen weight / MGI
- impaired glucose tolerance / MGI
- increased systemic arterial diastolic blood pressure / MGI
- increased systemic arterial systolic blood pressure / MGI
- impaired gastric peristalsis / MGI
- decreased susceptibility to weight gain / MGI
- increased lung compliance / MGI