MGI phenotypes (gene matching)
B6.129-Krt36tm1Hpt/H
| Status | Available to order |
| EMMA ID | EM:04832 |
| Citation information | RRID:IMSR_EM:04832 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Krt36tm1Hpt/H |
| Alternative name | Krt36 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Krt36tm1Hpt |
| Gene/Transgene symbol | Krt36 |
Information from provider
| Provider | Heiko Peters |
| Provider affiliation | Institute of Human Genetics, Newcastle University |
| Genetic information | Targeted mutation of Krt36, a gene encoding an acidic keratin. A loxP-flanked cassette (pMM-neoflox8; Ref. Kraus et al., 2001; PubMed-ID: 11514602) was used to replace Krt36 exons 1-3 (pos. 99966914 - 99964901 on mouse chromosome 11; RefSeq: NC_000077). The neo-cassette can be removed by crossing to Cre-expressing mouse strains. However, Cre-mediated removal of the neo cassette results in a weaker phenotype due to the expression of a partially functional, hybrid Krt36 gene (probably caused by splicing from a neighboring Krt gene). In contrast, Krt36 mRNA is undetectable in homozygous Krt36 mutants containing the neo cassette (i.e. most likely representing a null allele) and it is therefore recommended not to excise the neo cassette. |
| Phenotypic information | Hyperkeratosis of scales (tail) and filiform papillae (tongue). The analyses are not yet complete but if present, the phenotype is very weak in heterozygotes. An obvious hyperkeratosis is however apparent in homozygous mutant animals. |
| Breeding history | Crossed to C57BL/6 for 7 generations (N7) and subsequently maintained by intercrossing. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous C57BL/6 males |
| Breeding at archiving centre | Males archived upon arrival at the archiving centre. No breeding performed at the archiving centre. |