C57BL/6N-Tg(NES/TK-PDGFB,-lacZ)312Kfn/Kctt
| Status | Available to order |
| EMMA ID | EM:04887 |
| Citation information | RRID:IMSR_EM:04887 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Tg(NES/TK-PDGFB,-lacZ)312Kfn/Kctt |
| Alternative name | UB6n-Nestin-PDGF B-LacZ |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(NES/TK-PDGFB,-lacZ)312Kfn |
| Gene/Transgene symbol | Tg(NES/TK-PDGFB,-lacZ)312Kfn |
Information from provider
| Provider | Karin Forsberg Nilsson |
| Provider affiliation | Dept of Genetics and Pathology, Uppsala University |
| Genetic information | pTG8-nes/tk-Pdgfb-lacZ: The mice express 0.7 kb human PDGF-B cDNA under control of the nestin 2nd intron minimal enhancer for expression in embryonic neural stem cells and the TK promoter; IRES sequences link PDGF-B to lacZ. |
| Phenotypic information | The NES/TK-PDGFB-lacZ mice are viable and fertile. In the developing brain, mice showed increased apoptosis in the developing striatum, which suggests a disturbed regulation of progenitor cells. Later in neurodevelopment, in early postnatal life, mice displayed enlarged lateral ventricles. This enlargement remained into adulthood and it was more pronounced in male mice than in transgenic female mice. Adult transgenic males showed behavioral aberrations and locomotor dysfunction. The retina is not analyzed in this strain. |
| Breeding history | Made in C57BL/6N oocytes and kept on C57BL/6N. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous males, wild-type females |
| Breeding at archiving centre | no breeding at Karolinska Inst. |
| Stage of embryos | 2-cell |
Literature references
- Enlarged lateral ventricles and aberrant behavior in mice overexpressing PDGF-B in embryonic neural stem cells.;Niklasson Mia, Bergström Tobias, Zhang Xiao-Qun, Gustafsdottir Sigrun M, Sjögren Maria, Edqvist Per-Henrik, Vennström Björn, Forsberg Maud, Forsberg-Nilsson Karin, ;2010;Experimental cell research;316;2779-89; 20643125