B6;129P2-Elavl1^tm1Dkon/Flmg

Status	Available to order
EMMA ID	EM:04960
Citation information	RRID:IMSR_EM:04960 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129P2-Elavl1^tm1Dkon/Flmg
Alternative name	Elavl1 fl/fl
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Elavl1^tm1Dkon
Gene/Transgene symbol	Elavl1

Information from provider

Provider	Dimitris KONTOYIANNIS
Provider affiliation	Immunology, B.S.R.C. Alexander Fleming
Genetic information	A targeting vector was designed to flank a 0.77-kb XbaI/EcoRI fragment containing the second exon of the Elavl1 gene with loxP sequences and placed adjacent to a loxP-flanked PMC-neo-pA neomycin resistance gene. 5' and 3' homology regions of 4.4 kb (EcoRI/XbaI) and 5.7 kb (EcoRI), respectively, were inserted on either side of the modified exon 2 sequences. Finally, the complete modified locus was subcloned at the 5' end of a herpes simplex virus thymidine kinase susceptibility gene for the negative selection of random integrants. Targeting procedures in 129/Ola embryonic stem cells and subsequent injections in C57BL/6 blastocysts for chimera production and germ-line transmission from three independent clones were performed using standard procedures.
Phenotypic information	This is a strain that can be used with tissue specific Cre expressing strains to induce Cre-mediated Elavl1 (HuR) ablation.
References	Control of thymic T cell maturation, deletion and egress by the RNA-binding protein HuR.;Papadaki Olympia, Milatos Stavros, Grammenoudi Sofia, Mukherjee Neelanjan, Keene Jack D, Kontoyiannis Dimitris L, ;2009;Journal of immunology (Baltimore, Md. : 1950);182;6779-88; 19454673 Divergent Innate and Epithelial Functions of the RNA-Binding Protein HuR in Intestinal Inflammation.;Christodoulou-Vafeiadou Eleni, Ioakeimidis Fotis, Andreadou Margarita, Giagkas Giorgos, Stamatakis George, Reczko Martin, Samiotaki Martina, Papanastasiou Anastasios D, Karakasiliotis Ioannis, Kontoyiannis Dimitris L, ;2018;Frontiers in immunology;9;2732; 30532756
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	homozygous C57BL/6 males, wild-type C57BL/6 females
Stage of embryos	8-cell

Disease and phenotype information

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
embryonic growth retardation / IMPC
edema / IMPC
abnormal embryo size / IMPC
increased circulating alanine transaminase level / IMPC
anophthalmia / IMPC
impaired pupillary reflex / IMPC
abnormal limb morphology / IMPC
decreased mean corpuscular hemoglobin / IMPC

MGI phenotypes (gene matching)

decreased bone marrow cell number / MGI
spleen hypoplasia / MGI
decreased trophoblast giant cell number / MGI
placental labyrinth hypoplasia / MGI
pale yolk sac / MGI
thymus hypoplasia / MGI
increased cellular sensitivity to gamma-irradiation / MGI
embryonic growth retardation / MGI
abnormal maternal decidual layer morphology / MGI
small placenta / MGI
abnormal chorionic plate morphology / MGI
abnormal placental labyrinth vasculature morphology / MGI
abnormal spongiotrophoblast cell morphology / MGI
lethality throughout fetal growth and development, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
embryonic lethality during organogenesis, incomplete penetrance / MGI
decreased spongiotrophoblast size / MGI

Literature references

Control of thymic T cell maturation, deletion and egress by the RNA-binding protein HuR.;Papadaki Olympia, Milatos Stavros, Grammenoudi Sofia, Mukherjee Neelanjan, Keene Jack D, Kontoyiannis Dimitris L, ;2009;Journal of immunology (Baltimore, Md. : 1950);182;6779-88; 19454673
Divergent Innate and Epithelial Functions of the RNA-Binding Protein HuR in Intestinal Inflammation.;Christodoulou-Vafeiadou Eleni, Ioakeimidis Fotis, Andreadou Margarita, Giagkas Giorgos, Stamatakis George, Reczko Martin, Samiotaki Martina, Papanastasiou Anastasios D, Karakasiliotis Ioannis, Kontoyiannis Dimitris L, ;2018;Frontiers in immunology;9;2732; 30532756

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6;129P2-Elavl1tm1Dkon/Flmg