B6.Cg-Enpp2^tm1.1Vart/Flmg

Status	Available to order
EMMA ID	EM:04968
Citation information	RRID:IMSR_EM:04968 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Enpp2^tm1.1Vart/Flmg
Alternative name	C57BL/6-Enpp2tm1.1Vart/Flmg
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Enpp2^tm1.1Vart
Gene/Transgene symbol	Enpp2

Information from provider

Provider	Vassilis Aidinis
Provider affiliation	Institute of Immunology, B.S.R.C. Al. Fleming
Genetic information	This allele was derived from Enpp2^tm1Vart, in which a neomycin selection cassette was inserted upstream of exon 1, and the neo cassette and exons 1 and 2 were flanked by loxP sites. Both exons and the selection cassette were then excised ubiquitously by CMV-cre-mediated recombination to generate the full knock-out allele. Heterozygous full KO mice are healthy and fertile. Homozygous full KO embryos die at E9.5 due to aberrant circulation and failure of neural tube closure. The absence of protein expression in homozygous full KO embryos was confirmed by immunohistochemistry.
Phenotypic information	Embryonic lethality before turning of embryo
References	ATX expression and LPA signalling are vital for the development of the nervous system.;Fotopoulou Stella, Oikonomou Nikos, Grigorieva Elena, Nikitopoulou Ioanna, Paparountas Triantafillos, Thanassopoulou Artemis, Zhao Zhenwen, Xu Yan, Kontoyiannis Dimitris L, Remboutsika Eumorphia, Aidinis Vassilis, ;2010;Developmental biology;339;451-64; 20079728
Homozygous fertile	not known
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous C57BL/6 males

Disease and phenotype information

MGI phenotypes (allele matching)

decreased cell proliferation / MGI
open neural tube / MGI
abnormal blood vessel morphology / MGI
abnormal vasculogenesis / MGI
irregular heartbeat / MGI
abnormal somite development / MGI
abnormal allantois morphology / MGI
abnormal blood circulation / MGI
abnormal neural tube morphology / MGI
abnormal vitelline vasculature morphology / MGI
abnormal axon extension / MGI
embryonic growth retardation / MGI
failure of initiation of embryo turning / MGI
abnormal chorionic plate morphology / MGI
abnormal trophoblast giant cell morphology / MGI
failure of chorioallantoic fusion / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
increased neural tube apoptosis / MGI

MGI phenotypes (gene matching)

decreased cell proliferation / MGI
open neural tube / MGI
wavy neural tube / MGI
abnormal blood vessel morphology / MGI
abnormal vasculogenesis / MGI
irregular heartbeat / MGI
abnormal somite development / MGI
decreased embryo size / MGI
absent vitelline blood vessels / MGI
abnormal allantois morphology / MGI
edema / MGI
abnormal blood circulation / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
no phenotypic analysis / MGI
abnormal vitelline vasculature morphology / MGI
abnormal forebrain development / MGI
kinked neural tube / MGI
abnormal axon extension / MGI
embryonic growth retardation / MGI
failure of initiation of embryo turning / MGI
dilated allantois / MGI
abnormal chorionic plate morphology / MGI
abnormal trophoblast giant cell morphology / MGI
decreased circulating tumor necrosis factor level / MGI
decreased circulating interleukin-6 level / MGI
abnormal chemokine level / MGI
decreased susceptibility to endotoxin shock / MGI
failure of chorioallantoic fusion / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
abnormal visceral endoderm morphology / MGI
increased neural tube apoptosis / MGI
abnormal visceral yolk sac endoderm morphology / MGI

Literature references

ATX expression and LPA signalling are vital for the development of the nervous system.;Fotopoulou Stella, Oikonomou Nikos, Grigorieva Elena, Nikitopoulou Ioanna, Paparountas Triantafillos, Thanassopoulou Artemis, Zhao Zhenwen, Xu Yan, Kontoyiannis Dimitris L, Remboutsika Eumorphia, Aidinis Vassilis, ;2010;Developmental biology;339;451-64; 20079728

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.Cg-Enpp2tm1.1Vart/Flmg