| Status | Available to order |
| EMMA ID | EM:04969 |
| Citation information | RRID:IMSR_EM:04969 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Elavl1tm1.1Dkon/Flmg |
| Alternative name | Elavl1+/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Elavl1tm1.2Dkon |
| Gene/Transgene symbol | Elavl1 |
| Provider | Dimitris KONTOYIANNIS |
| Provider affiliation | Immunology, B.S.R.C. Alexander Fleming |
| Genetic information | A targeting vector was designed to flank a 0.77-kb XbaI/EcoRI fragment containing the second exon of the Elavl1 gene with loxP sequences and placed adjacent to a loxP-flanked PMC-neo-pA neomycin resistance gene. 5' and 3' homology regions of 4.4 kb (EcoRI/XbaI) and 5.7 kb (EcoRI), respectively, were inserted on either side of the modified exon 2 sequences. Finally, the complete modified locus was subcloned at the 5' end of a herpes simplex virus thymidine kinase susceptibility gene for the negative selection of random integrants. Targeting procedures in 129/Ola embryonic stem cells and subsequent injections in C57BL/6 blastocysts for chimera production and germ-line transmission from three independent clones were performed using standard procedures. The HuR expression has been ablated by Cre excision of the targeted locus. |
| Phenotypic information | Null HuR exrpession in all tissues. Homozygous embryonic lethal. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous C57BL/6 males, wild-type C57BL/6 females |
| Stage of embryos | 2-cell |
