B6.Cg-Tg(Sat1)165bCwp/Kctt

Status	Available to order
EMMA ID	EM:04987
Citation information	RRID:IMSR_EM:04987 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(Sat1)165bCwp/Kctt
Alternative name	SAT4
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Sat1)165bCwp
Gene/Transgene symbol	Tg(Sat1)165bCwp

Information from provider

Provider	Leena Alhonen
Provider affiliation	Laboratory Animal Center, University of Eastern Finland
Genetic information	Transgene is the functional mouse spermidine/spermine N1-acetyltransferase (SSAT) gene under its own promoter. Integration in multiple (>20) copies in one locus. Ubiquitous expression of the transgene.
Phenotypic information	Multiple phenotypic changes including hairless and wrinkled skin, female infertility, shortened life span, hematological changes, altered glucose, energy and fat metabolism.
Breeding history	Transgenesis in BALB/c x DBA/2 background, later backcrossed to inbred C57BL/6 for many generations. Maintenance in C57BL/6JOlaHsd strain.
References	Activation of polyamine catabolism profoundly alters tissue polyamine pools and affects hair growth and female fertility in transgenic mice overexpressing spermidine/spermine N1-acetyltransferase.;Pietilä M, Alhonen L, Halmekytö M, Kanter P, Jänne J, Porter C W, ;1997;The Journal of biological chemistry;272;18746-51; 9228047 Relation of skin polyamines to the hairless phenotype in transgenic mice overexpressing spermidine/spermine N-acetyltransferase.;Pietilä M, Parkkinen J J, Alhonen L, Jänne J, ;2001;The Journal of investigative dermatology;116;801-5; 11348473 Overexpression of spermidine/spermine N-acetyltransferase in transgenic mice protects the animals from kainate-induced toxicity.;Kaasinen K, Koistinaho J, Alhonen L, Jänne J, ;2000;The European journal of neuroscience;12;540-8; 10712633 Spermidine/spermine N1-acetyltransferase overexpression in mice induces hypoactivity and spatial learning impairment.;Kaasinen Selma K, Oksman Mari, Alhonen Leena, Tanila Heikki, Jänne Juhani, ;2004;Pharmacology, biochemistry, and behavior;78;35-45; 15159132 Genetically altered expression of spermidine/spermine N1-acetyltransferase affects fat metabolism in mice via acetyl-CoA.;Jell Jason, Merali Salim, Hensen Mary L, Mazurchuk Richard, Spernyak Joseph A, Diegelman Paula, Kisiel Nicholas D, Barrero Carlos, Deeb Kristin K, Alhonen Leena, Patel Mulchand S, Porter Carl W, ;2007;The Journal of biological chemistry;282;8404-13; 17189273 Enhanced polyamine catabolism alters homeostatic control of white adipose tissue mass, energy expenditure, and glucose metabolism.;Pirinen Eija, Kuulasmaa Teemu, Pietilä Marko, Heikkinen Sami, Tusa Maija, Itkonen Paula, Boman Susanna, Skommer Joanna, Virkamäki Antti, Hohtola Esa, Kettunen Mikko, Fatrai Szabolcs, Kansanen Emilia, Koota Suvi, Niiranen Kirsi, Parkkinen Jyrki, Levonen Anna-Liisa, Ylä-Herttuala Seppo, Hiltunen J Kalervo, Alhonen Leena, Smith Ulf, Jänne Juhani, Laakso Markku, ;2007;Molecular and cellular biology;27;4953-67; 17485446 Activated polyamine catabolism leads to low cholesterol levels by enhancing bile acid synthesis.;Pirinen Eija, Gylling Helena, Itkonen Paula, Yaluri Nagendra, Heikkinen Sami, Pietilä Marko, Kuulasmaa Teemu, Tusa Maija, Cerrada-Gimenez Marc, Pihlajamäki Jussi, Alhonen Leena, Jänne Juhani, Miettinen Tatu A, Laakso Markku, ;2010;Amino acids;38;549-60; 19956992 Cutaneous application of alpha-methylspermidine activates the growth of resting hair follicles in mice.;Fashe Tekele M, Keinänen Tuomo A, Grigorenko Nikolay A, Khomutov Alex R, Jänne Juhani, Alhonen Leena, Pietilä Marko, ;2010;Amino acids;38;583-90; 19956989 Continuous oxidative stress due to activation of polyamine catabolism accelerates aging and protects against hepatotoxic insults.;Cerrada-Gimenez Marc, Pietilä Marko, Loimas Suvikki, Pirinen Eija, Hyvönen Mervi T, Keinänen Tuomo A, Jänne Juhani, Alhonen Leena, ;2011;Transgenic research;20;387-96; 20577801 Potent modulation of intestinal tumorigenesis in Apcmin/+ mice by the polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase.;Tucker Jody M, Murphy John T, Kisiel Nicholas, Diegelman Paula, Barbour Karen W, Davis Celestia, Medda Moussumi, Alhonen Leena, Jänne Juhani, Kramer Debora L, Porter Carl W, Berger Franklin G, ;2005;Cancer research;65;5390-8; 15958588 Activated polyamine catabolism depletes acetyl-CoA pools and suppresses prostate tumor growth in TRAMP mice.;Kee Kristin, Foster Barbara A, Merali Salim, Kramer Debora L, Hensen Mary L, Diegelman Paula, Kisiel Nicholas, Vujcic Slavoljub, Mazurchuk Richard V, Porter Carl W, ;2004;The Journal of biological chemistry;279;40076-83; 15252047 Overexpression of spermidine/spermine N1-acetyltransferase elevates the threshold to pentylenetetrazol-induced seizure activity in transgenic mice.;Kaasinen Selma K, Gröhn Olli H J, Keinänen Tuomo A, Alhonen Leena, Jänne Juhani, ;2003;Experimental neurology;183;645-52; 14552906
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	heterozygous males, wild-type C57BL/6J females
Breeding at archiving centre	No breeding at Karolinska Inst.
Stage of embryos	2-cell

Literature references

Activation of polyamine catabolism profoundly alters tissue polyamine pools and affects hair growth and female fertility in transgenic mice overexpressing spermidine/spermine N1-acetyltransferase.;Pietilä M, Alhonen L, Halmekytö M, Kanter P, Jänne J, Porter C W, ;1997;The Journal of biological chemistry;272;18746-51; 9228047
Relation of skin polyamines to the hairless phenotype in transgenic mice overexpressing spermidine/spermine N-acetyltransferase.;Pietilä M, Parkkinen J J, Alhonen L, Jänne J, ;2001;The Journal of investigative dermatology;116;801-5; 11348473
Overexpression of spermidine/spermine N-acetyltransferase in transgenic mice protects the animals from kainate-induced toxicity.;Kaasinen K, Koistinaho J, Alhonen L, Jänne J, ;2000;The European journal of neuroscience;12;540-8; 10712633
Spermidine/spermine N1-acetyltransferase overexpression in mice induces hypoactivity and spatial learning impairment.;Kaasinen Selma K, Oksman Mari, Alhonen Leena, Tanila Heikki, Jänne Juhani, ;2004;Pharmacology, biochemistry, and behavior;78;35-45; 15159132
Genetically altered expression of spermidine/spermine N1-acetyltransferase affects fat metabolism in mice via acetyl-CoA.;Jell Jason, Merali Salim, Hensen Mary L, Mazurchuk Richard, Spernyak Joseph A, Diegelman Paula, Kisiel Nicholas D, Barrero Carlos, Deeb Kristin K, Alhonen Leena, Patel Mulchand S, Porter Carl W, ;2007;The Journal of biological chemistry;282;8404-13; 17189273
Enhanced polyamine catabolism alters homeostatic control of white adipose tissue mass, energy expenditure, and glucose metabolism.;Pirinen Eija, Kuulasmaa Teemu, Pietilä Marko, Heikkinen Sami, Tusa Maija, Itkonen Paula, Boman Susanna, Skommer Joanna, Virkamäki Antti, Hohtola Esa, Kettunen Mikko, Fatrai Szabolcs, Kansanen Emilia, Koota Suvi, Niiranen Kirsi, Parkkinen Jyrki, Levonen Anna-Liisa, Ylä-Herttuala Seppo, Hiltunen J Kalervo, Alhonen Leena, Smith Ulf, Jänne Juhani, Laakso Markku, ;2007;Molecular and cellular biology;27;4953-67; 17485446
Activated polyamine catabolism leads to low cholesterol levels by enhancing bile acid synthesis.;Pirinen Eija, Gylling Helena, Itkonen Paula, Yaluri Nagendra, Heikkinen Sami, Pietilä Marko, Kuulasmaa Teemu, Tusa Maija, Cerrada-Gimenez Marc, Pihlajamäki Jussi, Alhonen Leena, Jänne Juhani, Miettinen Tatu A, Laakso Markku, ;2010;Amino acids;38;549-60; 19956992
Cutaneous application of alpha-methylspermidine activates the growth of resting hair follicles in mice.;Fashe Tekele M, Keinänen Tuomo A, Grigorenko Nikolay A, Khomutov Alex R, Jänne Juhani, Alhonen Leena, Pietilä Marko, ;2010;Amino acids;38;583-90; 19956989
Continuous oxidative stress due to activation of polyamine catabolism accelerates aging and protects against hepatotoxic insults.;Cerrada-Gimenez Marc, Pietilä Marko, Loimas Suvikki, Pirinen Eija, Hyvönen Mervi T, Keinänen Tuomo A, Jänne Juhani, Alhonen Leena, ;2011;Transgenic research;20;387-96; 20577801
Potent modulation of intestinal tumorigenesis in Apcmin/+ mice by the polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase.;Tucker Jody M, Murphy John T, Kisiel Nicholas, Diegelman Paula, Barbour Karen W, Davis Celestia, Medda Moussumi, Alhonen Leena, Jänne Juhani, Kramer Debora L, Porter Carl W, Berger Franklin G, ;2005;Cancer research;65;5390-8; 15958588
Activated polyamine catabolism depletes acetyl-CoA pools and suppresses prostate tumor growth in TRAMP mice.;Kee Kristin, Foster Barbara A, Merali Salim, Kramer Debora L, Hensen Mary L, Diegelman Paula, Kisiel Nicholas, Vujcic Slavoljub, Mazurchuk Richard V, Porter Carl W, ;2004;The Journal of biological chemistry;279;40076-83; 15252047
Overexpression of spermidine/spermine N1-acetyltransferase elevates the threshold to pentylenetetrazol-induced seizure activity in transgenic mice.;Kaasinen Selma K, Gröhn Olli H J, Keinänen Tuomo A, Alhonen Leena, Jänne Juhani, ;2003;Experimental neurology;183;645-52; 14552906

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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