| Status | Available to order |
| EMMA ID | EM:04988 |
| Citation information | RRID:IMSR_EM:04988 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129X1-Sat1tm1Alh/Kctt |
| Alternative name | SSAT-KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sat1tm1Alh |
| Gene/Transgene symbol | Sat1 |
| Provider | Leena Alhonen |
| Provider affiliation | Laboratory Animal Center, University of Eastern Finland |
| Genetic information | Mice have disrupted spermidine/spermine N1-acetyltransferase (SSAT) gene resulting in lost capability to catabolize polyamines via acetylation pathway. Endogenous X chromosomal mouse spermidine/spermine N1-acetyltransferase gene was disrupted by replacing part of exon 1 with neo gene. |
| Phenotypic information | Largely normal, insulin resistance at old age, altered susceptibility to ischemia-reperfusion or endotoxin induced tissue injury. These mice are an excellent counterpart to mice with SSAT overexpression. |
| Breeding history | Targeting in RW-4 ES cells injected into C57BL/6J blastocysts, mice later backcrossed to inbred C57BL/6 for more than 20 generations. Maintenance in C57BL/5JOlaHsd strain. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous males, wild-type C57BL/6J females |
| Breeding at archiving centre | No breeding at Karolinska Inst. |
| Stage of embryos | 2-cell |
