IMPC phenotypes (gene matching)
STOCK Cdkn2atm2.1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt
| Status | Available to order |
| EMMA ID | EM:04995 |
| Citation information | RRID:IMSR_EM:04995 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cdkn2atm2.1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt |
| Alternative name | Ctv-a INK4a-/- |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cnp-TVA,-lacZ)B8Ubc, Cdkn2atm2.1Rdp |
| Gene/Transgene symbol | Tg(Cnp-TVA,-lacZ)B8Ubc, Cdkn2a |
Information from provider
| Provider | Lene Uhrbom |
| Provider affiliation | Department of Neuroscience, Uppsala University |
| Additional owner | Lene Uhrbom, Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden |
| Genetic information | Transgenic construct: pCNPase-TV-A-IRES-lacZ made in FVB/N. Crossed with strain containing targeted deletion p16INK4a (exon 1a of the cyclin-dependent kinase inhibitor 2A gene). |
| Phenotypic information | Allows for infection of 2',3'-cyclic nucleotide 3' phosphodiesterase (CNPase)-expressing cells by RCAS virus. The deletion of exon 1a in the cyclin-dependent kinase inhibitor 2A gene results in complete loss of p16INK4a transcription but not p19ARF. |
| Breeding history | The TV-A founder mouse was crossed with FVB/N and subsequently intercrossed to establish homozygotes. Following, TVA-A transgenic mice were crossed with p16INK4a null mice and subsequently F1 progeny were crossed to generate mice homozygous for TV-A and p16INK4a deletion. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous males, wild-type FVB/N females |
| Breeding at archiving centre | No breeding at Karolinska Inst. |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- increased tumor incidence / MGI
- thymus hyperplasia / MGI
- increased sarcoma incidence / MGI
- increased osteosarcoma incidence / MGI
- increased incidence of tumors by chemical induction / MGI
- increased thymus weight / MGI
- increased double-positive T cell number / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- increased small lymphocytic lymphoma incidence / MGI
- increased splenocyte proliferation / MGI
- increased lymphoma incidence / MGI
MGI phenotypes (gene matching)
- extramedullary hematopoiesis / MGI
- abnormal spleen morphology / MGI
- thymus hyperplasia / MGI
- hindlimb paralysis / MGI
- abnormal retinal photoreceptor morphology / MGI
- increased metastatic potential / MGI
- microphthalmia / MGI
- abnormal lens morphology / MGI
- cataract / MGI
- abnormal retina morphology / MGI
- male infertility / MGI
- blindness / MGI
- neoplasm / MGI
- increased papilloma incidence / MGI
- abnormal tumor incidence / MGI
- increased tumor incidence / MGI
- increased B cell derived lymphoma incidence / MGI
- increased T cell derived lymphoma incidence / MGI
- increased sarcoma incidence / MGI
- increased fibrohistocytoma incidence / MGI
- increased carcinoma incidence / MGI
- seizures / MGI
- premature death / MGI
- abnormal eye morphology / MGI
- abnormal spleen red pulp morphology / MGI
- abnormal sperm number / MGI
- oligozoospermia / MGI
- no phenotypic analysis / MGI
- abnormal cell cycle / MGI
- retinal detachment / MGI
- testicular atrophy / MGI
- abnormal lens capsule morphology / MGI
- abnormal keratinocyte physiology / MGI
- increased hemangiosarcoma incidence / MGI
- increased tumor growth/size / MGI
- abnormal retinal inner nuclear layer morphology / MGI
- increased osteosarcoma incidence / MGI
- increased squamous cell carcinoma incidence / MGI
- increased incidence of tumors by chemical induction / MGI
- increased incidence of tumors by ionizing radiation induction / MGI
- decreased incidence of tumors by chemical induction / MGI
- increased testis weight / MGI
- decreased testis weight / MGI
- increased thymus weight / MGI
- increased double-positive T cell number / MGI
- abnormal posterior eye segment morphology / MGI
- abnormal retinal ganglion layer morphology / MGI
- reproductive system phenotype / MGI
- vision/eye phenotype / MGI
- abnormal skin physiology / MGI
- abnormal lens development / MGI
- abnormal cell physiology / MGI
- abnormal retinal neuronal layer morphology / MGI
- binocular blindness / MGI
- abnormal spermatocyte morphology / MGI
- delayed cellular replicative senescence / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- increased spleen white pulp amount / MGI
- increased lung carcinoma incidence / MGI
- increased brain tumor incidence / MGI
- increased small lymphocytic lymphoma incidence / MGI
- increased histiocytic sarcoma incidence / MGI
- increased splenocyte proliferation / MGI
- increased gastrointestinal stromal tumor incidence / MGI
- primary vitreous hyperplasia / MGI
- increased melanoma incidence / MGI
- increased cutaneous melanoma incidence / MGI
- increased acute lymphoblastic leukemia incidence / MGI
- increased fibrosarcoma incidence / MGI
- persistent hyperplastic primary vitreous / MGI
- increased fibroblast proliferation / MGI
- increased lymphoma incidence / MGI
Literature references
- Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
- Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis.;Sharpless N E, Bardeesy N, Lee K H, Carrasco D, Castrillon D H, Aguirre A J, Wu E A, Horner J W, DePinho R A, ;2001;Nature;413;86-91; 11544531