IMPC phenotypes (gene matching)
STOCK Cdkn2atm1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt
| Status | Available to order |
| EMMA ID | EM:04997 |
| Citation information | RRID:IMSR_EM:04997 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cdkn2atm1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt |
| Alternative name | Ctv-a Ink4a-Arf-/- |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cnp-TVA,-lacZ)B8Ubc, Cdkn2atm1Rdp |
| Gene/Transgene symbol | Tg(Cnp-TVA,-lacZ)B8Ubc, Cdkn2a |
Information from provider
| Provider | Lene Uhrbom |
| Provider affiliation | Department of Neuroscience, Uppsala University |
| Additional owner | Lene Uhrbom, Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden |
| Genetic information | Transgenic construct: pCNPase-TV-A-IRES-lacZ made in FVB/N. Crossed with strain containing targeted deletion p16Ink4a and p19Arf (exons 2 and 3 of the cyclin-dependent kinase inhibitor 2a gene). |
| Phenotypic information | Allows for infection of 2',3'-cyclic nucleotide 3' phosphodiesterase (CNPase)-expressing cells by RCAS virus. The deletion of exons 2 and 3 in the cyclin-dependent kinase inhibitor 2a gene results in complete loss of both p16Ink4a and p19Arf. |
| Breeding history | The TV-A founder mouse was crossed with FVB/N and subsequently intercrossed to establish homozygotes. The p16Ink4a-p19Arf null mice on C57BL/6 background were previously crossed with another transgenic mouse on FVB/N background. These were crossed with FVB/N for two generations to isolate the p16Ink4a-p19Arf genotype solely and then intercrossed once to generate p16Ink4a-p19Arf-/- mice. Following, TV-A transgenic mice were crossed with p16Ink4a-p19Arf null mice and subsequently F1 progeny were crossed to generate mice homozygous for TV-A and p16Ink4a-p19Arf deletion. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous males, wild-type FVB/N females |
| Breeding at archiving centre | no breeding at Karolinska Inst. |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- abnormal retinal photoreceptor morphology / MGI
- microphthalmia / MGI
- cataract / MGI
- abnormal posterior eye segment morphology / MGI
- abnormal lens development / MGI
- abnormal retinal neuronal layer morphology / MGI
- persistent hyperplastic primary vitreous / MGI
- increased brain tumor incidence / MGI
- extramedullary hematopoiesis / MGI
- abnormal spleen morphology / MGI
- increased tumor incidence / MGI
- increased B cell derived lymphoma incidence / MGI
- increased sarcoma incidence / MGI
- abnormal spleen red pulp morphology / MGI
- abnormal cell cycle / MGI
- increased hemangiosarcoma incidence / MGI
- increased spleen white pulp amount / MGI
- increased cutaneous melanoma incidence / MGI
- increased fibrosarcoma incidence / MGI
- increased fibroblast proliferation / MGI
- abnormal keratinocyte physiology / MGI
- increased squamous cell carcinoma incidence / MGI
- abnormal skin physiology / MGI
- abnormal cell physiology / MGI
- delayed cellular replicative senescence / MGI
- premature death / MGI
- increased lymphoma incidence / MGI
MGI phenotypes (gene matching)
- extramedullary hematopoiesis / MGI
- abnormal spleen morphology / MGI
- thymus hyperplasia / MGI
- hindlimb paralysis / MGI
- abnormal retinal photoreceptor morphology / MGI
- increased metastatic potential / MGI
- microphthalmia / MGI
- abnormal lens morphology / MGI
- cataract / MGI
- abnormal retina morphology / MGI
- male infertility / MGI
- blindness / MGI
- neoplasm / MGI
- increased papilloma incidence / MGI
- abnormal tumor incidence / MGI
- increased tumor incidence / MGI
- increased B cell derived lymphoma incidence / MGI
- increased T cell derived lymphoma incidence / MGI
- increased sarcoma incidence / MGI
- increased fibrohistocytoma incidence / MGI
- increased carcinoma incidence / MGI
- seizures / MGI
- premature death / MGI
- abnormal eye morphology / MGI
- abnormal spleen red pulp morphology / MGI
- abnormal sperm number / MGI
- oligozoospermia / MGI
- no phenotypic analysis / MGI
- abnormal cell cycle / MGI
- retinal detachment / MGI
- testicular atrophy / MGI
- abnormal lens capsule morphology / MGI
- abnormal keratinocyte physiology / MGI
- increased hemangiosarcoma incidence / MGI
- increased tumor growth/size / MGI
- abnormal retinal inner nuclear layer morphology / MGI
- increased osteosarcoma incidence / MGI
- increased squamous cell carcinoma incidence / MGI
- increased incidence of tumors by chemical induction / MGI
- increased incidence of tumors by ionizing radiation induction / MGI
- decreased incidence of tumors by chemical induction / MGI
- increased testis weight / MGI
- decreased testis weight / MGI
- increased thymus weight / MGI
- increased double-positive T cell number / MGI
- abnormal posterior eye segment morphology / MGI
- abnormal retinal ganglion layer morphology / MGI
- reproductive system phenotype / MGI
- vision/eye phenotype / MGI
- abnormal skin physiology / MGI
- abnormal lens development / MGI
- abnormal cell physiology / MGI
- abnormal retinal neuronal layer morphology / MGI
- binocular blindness / MGI
- abnormal spermatocyte morphology / MGI
- delayed cellular replicative senescence / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- increased spleen white pulp amount / MGI
- increased lung carcinoma incidence / MGI
- increased brain tumor incidence / MGI
- increased small lymphocytic lymphoma incidence / MGI
- increased histiocytic sarcoma incidence / MGI
- increased splenocyte proliferation / MGI
- increased gastrointestinal stromal tumor incidence / MGI
- primary vitreous hyperplasia / MGI
- increased melanoma incidence / MGI
- increased cutaneous melanoma incidence / MGI
- increased acute lymphoblastic leukemia incidence / MGI
- increased fibrosarcoma incidence / MGI
- persistent hyperplastic primary vitreous / MGI
- increased fibroblast proliferation / MGI
- increased lymphoma incidence / MGI
Literature references
- Role of the INK4a locus in tumor suppression and cell mortality.;Serrano M, Lee H, Chin L, Cordon-Cardo C, Beach D, DePinho R A, ;1996;Cell;85;27-37; 8620534
- Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151