B6.129P2-Tln1^tm4.1Crit/CritH

Status	Available to order
EMMA ID	EM:04999
Citation information	RRID:IMSR_EM:04999 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Tln1^tm4.1Crit/CritH
Alternative name	Tln1(tm4.1Crit)
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Tln1^tm4.1Crit
Gene/Transgene symbol	Tln1

Information from provider

Provider	Sue Monkley
Provider affiliation	Biomedical Services, University of Leicester
Additional owner	Prof David Critchley, Dept of Biochemistry, University of Leicester, Leicester, UK
Genetic information	Conditional talin 1 allele with loxP sites upstream of the 1st coding exon and downstream of the 4th coding exon. Cre-mediated deletion of exons 1-4 results in a null allele.
Phenotypic information	The homozygous floxed Tln1 mice are viable and fertile and do not exhibit evidence of any phenotype.
Breeding history	The line was generated with a floxed neo cassette that was deleted by crossing to cre-deleter mice. The allele was then backcrossed to C57BL/6 and then intercrossed to generate homozygous mice. The allele is maintained as homozygous.
References	Talin is required for integrin-mediated platelet function in hemostasis and thrombosis.;Petrich Brian G, Marchese Patrizia, Ruggeri Zaverio M, Spiess Saskia, Weichert Rachel A M, Ye Feng, Tiedt Ralph, Skoda Radek C, Monkley Susan J, Critchley David R, Ginsberg Mark H, ;2007;The Journal of experimental medicine;204;3103-11; 18086863 Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo.;Nieswandt Bernhard, Moser Markus, Pleines Irina, Varga-Szabo David, Monkley Sue, Critchley David, Fässler Reinhard, ;2007;The Journal of experimental medicine;204;3113-8; 18086864 Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions.;Conti Francesco J, Monkley Sue J, Wood Malcolm R, Critchley David R, Müller Ulrich, ;2009;Development (Cambridge, England);136;3597-606; 19793892
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6.129/Sv males
Breeding at archiving centre	Males were archived upon arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

MGI phenotypes (gene matching)

abnormal embryo development / MGI
abnormal ectoderm development / MGI
abnormal mesoderm development / MGI
decreased embryo size / MGI
embryonic growth arrest / MGI
abnormal embryonic tissue morphology / MGI
abnormal extraembryonic tissue morphology / MGI
abnormal gastrulation movements / MGI
disorganized extraembryonic tissue / MGI
abnormal trophoblast layer morphology / MGI
abnormal extraembryonic mesoderm development / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
abnormal proamniotic cavity morphology / MGI
abnormal amniotic cavity morphology / MGI
absent ectoplacental cavity / MGI
small proamniotic cavity / MGI
absent extraembryonic coelom / MGI
small amniotic cavity / MGI
embryonic-extraembryonic boundary constriction / MGI

Literature references

Talin is required for integrin-mediated platelet function in hemostasis and thrombosis.;Petrich Brian G, Marchese Patrizia, Ruggeri Zaverio M, Spiess Saskia, Weichert Rachel A M, Ye Feng, Tiedt Ralph, Skoda Radek C, Monkley Susan J, Critchley David R, Ginsberg Mark H, ;2007;The Journal of experimental medicine;204;3103-11; 18086863
Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo.;Nieswandt Bernhard, Moser Markus, Pleines Irina, Varga-Szabo David, Monkley Sue, Critchley David, Fässler Reinhard, ;2007;The Journal of experimental medicine;204;3113-8; 18086864
Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions.;Conti Francesco J, Monkley Sue J, Wood Malcolm R, Critchley David R, Müller Ulrich, ;2009;Development (Cambridge, England);136;3597-606; 19793892

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.129P2-Tln1tm4.1Crit/CritH