MGI phenotypes (allele matching)
B6.129P2-Tln2tm2Crit/CritH
| Status | Available to order |
| EMMA ID | EM:05000 |
| Citation information | RRID:IMSR_EM:05000 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Tln2tm2Crit/CritH |
| Alternative name | Tln2(cd) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Tln2tm2Crit |
| Gene/Transgene symbol | Tln2 |
Information from provider
| Provider | Sue Monkley |
| Provider affiliation | Biomedical Services, University of Leicester |
| Additional owner | Prof David Critchley, Dept of Biochemitry, University of Leicester, Leicester, UK |
| Genetic information | Using a 2-step targeting strategy and loxP sites the whole coding sequence of the Tln2 gene was deleted (~200kb). |
| Phenotypic information | The homozygous Tln2cd mice are viable and fertile and do not exhibit evidence of any phenotype. |
| Breeding history | The line was generated with a floxed Neo cassette that was deleted by crossing to cre-deleter mice. The allele was then backcrossed to C57BL/6 and then intercrossed to generate homozygous mice. The allele is maintained as homozygous. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous C57BL/6.129/Sv males |
| Breeding at archiving centre | Males were archived at the time of arrival. No breeding was performed at the archiving centre. |
Disease and phenotype information
Literature references
- Talin is required for integrin-mediated platelet function in hemostasis and thrombosis.;Petrich Brian G, Marchese Patrizia, Ruggeri Zaverio M, Spiess Saskia, Weichert Rachel A M, Ye Feng, Tiedt Ralph, Skoda Radek C, Monkley Susan J, Critchley David R, Ginsberg Mark H, ;2007;The Journal of experimental medicine;204;3103-11; 18086863
- Loss of talin1 in platelets abrogates integrin activation, platelet aggregation, and thrombus formation in vitro and in vivo.;Nieswandt Bernhard, Moser Markus, Pleines Irina, Varga-Szabo David, Monkley Sue, Critchley David, Fässler Reinhard, ;2007;The Journal of experimental medicine;204;3113-8; 18086864
- Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions.;Conti Francesco J, Monkley Sue J, Wood Malcolm R, Critchley David R, Müller Ulrich, ;2009;Development (Cambridge, England);136;3597-606; 19793892