B6;CBA-Bnc2^{Gt(pU21)18Imeg}/Orl

Status	Available to order
EMMA ID	EM:05043
Citation information	RRID:IMSR_EM:05043 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;CBA-Bnc2^{Gt(pU21)18Imeg}/Orl
Alternative name	Ayu21-18
Strain type	Gene-trap
Allele/Transgene symbol	Bnc2^{Gt(pU21)18Imeg}
Gene/Transgene symbol	Bnc2

Information from provider

Provider	Philippe Djian
Provider affiliation	CNRS/Université Paris Descartes
Additional owner	Dr. Kimi Araki, Institute of Molecular Embryology and Genetics, Kumamoto University, Japan
Genetic information	Insertion of the gene trap vector pU21 in the intron separating exons 2a and 3 of the Bnc2 gene. Disruption of the gene resulting in inactivation.
Phenotypic information	Neonatal lethality in the homozygous mutant with palatal cleft and craniofacial bone abnormalities.
References	Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins.;Vanhoutteghem Amandine, Maciejewski-Duval Anna, Bouche Cyril, Delhomme Brigitte, Hervé Françoise, Daubigney Fabrice, Soubigou Guillaume, Araki Masatake, Araki Kimi, Yamamura Ken-ichi, Djian Philippe, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;14432-7; 19706529
Homozygous fertile	no
Homozygous viable	no
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	heterozygous C57BL/6 x CBA males, wild-type (C57BL/6 x CBA)F1 females

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Posterior urethral valve / Orphanet_93110

IMPC phenotypes (gene matching)

decreased fluid intake / IMPC
decreased exploration in new environment / IMPC
increased cornea thickness / IMPC

MGI phenotypes (allele matching)

microcephaly / MGI
parietal bone hypoplasia / MGI
abnormal pterygoid process morphology / MGI
small alisphenoid bone / MGI
absent palatine bone / MGI
abnormal maxillary shelf morphology / MGI
frontal bone hypoplasia / MGI
meteorism / MGI
abnormal palate development / MGI
decreased birth body size / MGI
palatal shelves fail to meet at midline / MGI
cleft secondary palate / MGI
decreased tongue size / MGI
neonatal lethality, complete penetrance / MGI
wide sagittal suture / MGI
large posterior fontanelle / MGI

MGI phenotypes (gene matching)

microcephaly / MGI
parietal bone hypoplasia / MGI
abnormal pterygoid process morphology / MGI
small alisphenoid bone / MGI
absent palatine bone / MGI
abnormal maxillary shelf morphology / MGI
frontal bone hypoplasia / MGI
meteorism / MGI
abnormal palate development / MGI
decreased birth body size / MGI
palatal shelves fail to meet at midline / MGI
cleft secondary palate / MGI
decreased tongue size / MGI
neonatal lethality, complete penetrance / MGI
wide sagittal suture / MGI
large posterior fontanelle / MGI

Literature references

Basonuclin 2 has a function in the multiplication of embryonic craniofacial mesenchymal cells and is orthologous to disco proteins.;Vanhoutteghem Amandine, Maciejewski-Duval Anna, Bouche Cyril, Delhomme Brigitte, Hervé Françoise, Daubigney Fabrice, Soubigou Guillaume, Araki Masatake, Araki Kimi, Yamamura Ken-ichi, Djian Philippe, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;14432-7; 19706529
Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis.;Bobowski-Gerard Marie, Boulet Clémence, Zummo Francesco P, Dubois-Chevalier Julie, Gheeraert Céline, Bou Saleh Mohamed, Strub Jean-Marc, Farce Amaury, Ploton Maheul, Guille Loïc, Vandel Jimmy, Bongiovanni Antonino, Very Ninon, Woitrain Eloïse, Deprince Audrey, Lalloyer Fanny, Bauge Eric, Ferri Lise, Ntandja-Wandji Line-Carolle, Cotte Alexia K, Grangette Corinne, Vallez Emmanuelle, Cianférani Sarah, Raverdy Violeta, Caiazzo Robert, Gnemmi Viviane, Leteurtre Emmanuelle, Pourcet Benoit, Paumelle Réjane, Ravnskjaer Kim, Lassailly Guillaume, Haas Joel T, Mathurin Philippe, Pattou François, Dubuquoy Laurent, Staels Bart, Lefebvre Philippe, Eeckhoute Jérôme, ;2022;Nature communications;13;5324; 36088459

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.

B6;CBA-Bnc2Gt(pU21)18Imeg/Orl