B6.Cg-Tg(Tyr-NRAS*Q61K)1Bee/Orl
| Status | Available to order |
| EMMA ID | EM:05143 |
| Citation information | RRID:IMSR_EM:05143 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Tyr-NRAS*Q61K)1Bee/Orl |
| Alternative name | Tyr::NrasQ61K |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Tyr-NRAS*Q61K)1Bee |
| Gene/Transgene symbol | Tg(Tyr-NRAS*Q61K)1Bee |
Information from provider
| Provider | Friedrich Beermann |
| Provider affiliation | EPFL SV ISREC |
| Genetic information | Mouse line expressing a human oncogenic Nras (NrasQ61K) in the melanocyte lineage. |
| Phenotypic information | All transgenics are hyperpigmented in paws, coat, ears and tails. There is a low incidence of melanoma with long latency (less than 30%, 1-1.5 years); however, melanoma will develop in breeding to e.g. mice deficient for INK4a. |
| Breeding history | Mice are backcrossed at least 10x to C57BL |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males, wild-type C57BL/6J females |
Literature references
- Metastasizing melanoma formation caused by expression of activated N-RasQ61K on an INK4a-deficient background.;Ackermann Julien, Frutschi Manon, Kaloulis Kostas, McKee Thomas, Trumpp Andreas, Beermann Friedrich, ;2005;Cancer research;65;4005-11; 15899789
- Beta-catenin induces immortalization of melanocytes by suppressing p16INK4a expression and cooperates with N-Ras in melanoma development.;Delmas Véronique, Beermann Friedrich, Martinozzi Silvia, Carreira Suzanne, Ackermann Julien, Kumasaka Mayuko, Denat Laurence, Goodall Jane, Luciani Flavie, Viros Amaya, Demirkan Nese, Bastian Boris C, Goding Colin R, Larue Lionel, ;2007;Genes & development;21;2923-35; 18006687
- The fibroblast growth factor-2 is not essential for melanoma formation in a transgenic mouse model.;Ackermann Julien, Beermann Friedrich, ;2005;Pigment cell research;18;315-9; 16029424
- Differential roles of the pRb and Arf/p53 pathways in murine naevus and melanoma genesis.;Ferguson Blake, Konrad Muller H, Handoko Herlina Y, Khosrotehrani Kiarash, Beermann Friedrich, Hacker Elke, Soyer H Peter, Bosenberg Marcus, Walker Graeme J, ;2010;Pigment cell & melanoma research;23;771-80; 20718941
- Murine neonatal melanocytes exhibit a heightened proliferative response to ultraviolet radiation and migrate to the epidermal basal layer.;Walker Graeme J, Kimlin Michael G, Hacker Elke, Ravishankar Sugandha, Muller H Konrad, Beermann Friedrich, Hayward Nicholas K, ;2009;The Journal of investigative dermatology;129;184-93; 18633434
- RNA Therapy for Oncogenic NRAS-Driven Nevi Induces Apoptosis.;Bryant Dale, Barberan-Martin Sara, Maeshima Ruhina, Del Valle Torres Ignacio, Rabii Mohammad, Baird William, Sauvadet Aimie, Demetriou Charalambos, Jones Phoebe, Knöpfel Nicole, Michailidis Fanourios, Riachi Melissa, Bennett Dorothy C, Zecchin Davide, Pittman Alan, Polubothu Satyamaanasa, Hart Stephen, Kinsler Veronica A, ;2025;The Journal of investigative dermatology;145;122-134.e11; 38897541