B6.129S-Ctse^129S/SvHsd/H

Status	Available to order
EMMA ID	EM:05213
Citation information	RRID:IMSR_EM:05213 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129S-Ctse^129S/SvHsd/H
Alternative name	C57Bl_CTSEhigh
Strain type	Spontaneous
Allele/Transgene symbol	Ctse^129S/SvHsd
Gene/Transgene symbol	Ctse

Information from provider

Provider	Benny Chain
Provider affiliation	CBU, UCL
Genetic information	Cathepsin E is expressed in both haemopoietic and non-haemopoietic tissue. We previously described a natural mutation in the cathepsin E promoter (see reference below) which confers a low expressor phenotype in haemopoietic tissue of C57BL strains. Since this strain is widely used for immunological studies, we have backcrossed the cathepsin E high allele from 129/Sv mice into the C57BL/6J background for eight generations.
Phenotypic information	Mice breed normally. No obvious phenotype has been described as yet but cathepsin E has been implicated in regulation of antigen processing, and other aspects of innate immunity. The mice are maintained on 129/Sv background as well as C57BL/6 because there is a natural polymorphism which makes C57BL/6 mice a low expressing strain.
Breeding history	129/Sv mouse was backcrossed repeatedly onto the C57BL/6J background. At each generation cathepsin E high genotype was selected for next generation backcross. After 8 generations the mice are maintained by inbreeding of mice with the cathepsin E high allele on a C57BL/6J background.
References	The expression and function of cathepsin E in dendritic cells.;Chain Benjamin M, Free Paul, Medd Patrick, Swetman Claire, Tabor Alethea B, Terrazzini Nadia, ;2005;Journal of immunology (Baltimore, Md. : 1950);174;1791-800; 15699105 Natural cathepsin E deficiency in the immune system of C57BL/6J mice.;Tulone Calogero, Tsang Jhen, Prokopowicz Zofia, Grosvenor Nicholas, Chain Benny, ;2007;Immunogenetics;59;927-35; 18000662
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous C57BL/6 males
Breeding at archiving centre	Males were sperm frozen upon arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

MGI phenotypes (gene matching)

alopecia / MGI
reddish skin / MGI
dermatitis / MGI
skin lesions / MGI
thick epidermis / MGI
epidermal hyperplasia / MGI
mixed cellular infiltration to dermis / MGI
abnormal immune system physiology / MGI
abnormal dendritic cell physiology / MGI
abnormal dendritic cell antigen presentation / MGI
increased IgE level / MGI
increased eosinophil cell number / MGI
increased double-positive T cell number / MGI
reproductive system phenotype / MGI
increased circulating interleukin-18 level / MGI
increased circulating interleukin-1 beta level / MGI
increased interleukin-4 secretion / MGI
increased interleukin-5 secretion / MGI

Literature references

The expression and function of cathepsin E in dendritic cells.;Chain Benjamin M, Free Paul, Medd Patrick, Swetman Claire, Tabor Alethea B, Terrazzini Nadia, ;2005;Journal of immunology (Baltimore, Md. : 1950);174;1791-800; 15699105
Natural cathepsin E deficiency in the immune system of C57BL/6J mice.;Tulone Calogero, Tsang Jhen, Prokopowicz Zofia, Grosvenor Nicholas, Chain Benny, ;2007;Immunogenetics;59;927-35; 18000662

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
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B6.129S-Ctse129S/SvHsd/H