B6.129P2-Iapp^tm1Sgm/Kctt

Status	Available to order
EMMA ID	EM:05269
Citation information	RRID:IMSR_EM:05269 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Iapp^tm1Sgm/Kctt
Alternative name	Iapp knock-out mice
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Iapp^tm1Sgm
Gene/Transgene symbol	Iapp

Information from provider

Provider	Samuel Gebre-Medhin
Provider affiliation	Experimental Medical Science, Lund University
Genetic information	Targeting construct is derived from BALB/c DNA. Entire coding DNA sequence for mature IAPP is replaced by neomycin resistance gene.
Phenotypic information	Augmented insulin secretion. Reduced nociception. Reduced bone mineral density.
Breeding history	8 backcrosses to C57BL.
References	Increased insulin secretion and glucose tolerance in mice lacking islet amyloid polypeptide (amylin).;Gebre-Medhin S, Mulder H, Pekny M, Westermark G, Törnell J, Westermark P, Sundler F, Ahrén B, Betsholtz C, ;1998;Biochemical and biophysical research communications;250;271-7; 9753619 Reduced nociceptive behavior in islet amyloid polypeptide (amylin) knockout mice.;Gebre-Medhin S, Mulder H, Zhang Y, Sundler F, Betsholtz C, ;1998;Brain research. Molecular brain research;63;180-3; 9838101 Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo.;Dacquin Romain, Davey Rachel A, Laplace Catherine, Levasseur Régis, Morris Howard A, Goldring Steven R, Gebre-Medhin Samuel, Galson Deborah L, Zajac Jeffrey D, Karsenty Gérard, ;2004;The Journal of cell biology;164;509-14; 14970190
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden
Animals used for archiving	homozygous males, heterozygous C57BL/6J females
Breeding at archiving centre	No breeding at Karolinska Inst.
Stage of embryos	2-cell

Disease and phenotype information

MGI phenotypes (allele matching)

decreased bone mineral density / MGI
abnormal trabecular bone morphology / MGI
decreased compact bone thickness / MGI
decreased bone mass / MGI
increased osteoclast cell number / MGI
increased bone resorption / MGI
osteoporosis / MGI
increased body weight / MGI
increased circulating insulin level / MGI
improved glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
osteoporosis / MGI
abnormal trabecular bone morphology / MGI
decreased compact bone thickness / MGI
increased body weight / MGI
increased circulating insulin level / MGI
decreased bone mass / MGI
increased osteoclast cell number / MGI
increased bone resorption / MGI
improved glucose tolerance / MGI
homeostasis/metabolism phenotype / MGI

Literature references

Increased insulin secretion and glucose tolerance in mice lacking islet amyloid polypeptide (amylin).;Gebre-Medhin S, Mulder H, Pekny M, Westermark G, Törnell J, Westermark P, Sundler F, Ahrén B, Betsholtz C, ;1998;Biochemical and biophysical research communications;250;271-7; 9753619
Reduced nociceptive behavior in islet amyloid polypeptide (amylin) knockout mice.;Gebre-Medhin S, Mulder H, Zhang Y, Sundler F, Betsholtz C, ;1998;Brain research. Molecular brain research;63;180-3; 9838101
Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo.;Dacquin Romain, Davey Rachel A, Laplace Catherine, Levasseur Régis, Morris Howard A, Goldring Steven R, Gebre-Medhin Samuel, Galson Deborah L, Zajac Jeffrey D, Karsenty Gérard, ;2004;The Journal of cell biology;164;509-14; 14970190
Amylin-Calcitonin receptor signaling in the medial preoptic area mediates affiliative social behaviors in female mice.;Fukumitsu Kansai, Kaneko Misato, Maruyama Teppo, Yoshihara Chihiro, Huang Arthur J, McHugh Thomas J, Itohara Shigeyoshi, Tanaka Minoru, Kuroda Kumi O, ;2022;Nature communications;13;709; 35136064
Calcitonin receptor signaling in the medial preoptic area enables risk-taking maternal care.;Yoshihara Chihiro, Tokita Kenichi, Maruyama Teppo, Kaneko Misato, Tsuneoka Yousuke, Fukumitsu Kansai, Miyazawa Eri, Shinozuka Kazutaka, Huang Arthur J, Nishimori Katsuhiko, McHugh Thomas J, Tanaka Minoru, Itohara Shigeyoshi, Touhara Kazushige, Miyamichi Kazunari, Kuroda Kumi O, ;2021;Cell reports;35;109204; 34077719

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Iapptm1Sgm/Kctt