| Status | Available to order |
| EMMA ID | EM:05273 |
| Citation information | RRID:IMSR_EM:05273 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Lrp1tm4Ajmr/Orl |
| Alternative name | Lrp1 distal NPXYXXL (NPXY2) motif knock-in mutant |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Lrp1tm4Ajmr |
| Gene/Transgene symbol | Lrp1 |
| Provider | Anton Roebroek |
| Provider affiliation | Human Genetics, K.U. Leuven |
| Genetic information | Lrp1 knock-in mouse carrying mutant Lrp1 cDNA sequences in the 3' end of the gene replacing the corresponding genomic sequences: no intron 76 to intron 88 sequences present. The mutations are introduced to inactivate the distal NPXYXXL (NPXY2) motif in the intracellular domain of LRP1 (NPVYATL -> AAVAATL). The Lrp1 knock-in allele was generated by application of recombinase mediated cassette exchange (RMCE) in a ES cell line carrying an exchangeable cassette with a selection marker in the Lrp1 gene replacing the 3' end exons (exons 76 to 89) of the gene. By application of RMCE the Lrp1 gene was functionally restored by wild-type or mutant Lrp1 cDNA sequences resulting in a series of Lrp1 knock-in mutant alleles and mice including the Lrp1 distal NPXYXXL (NPXY2) motif knock-in mutant. |
| Phenotypic information | 1. Initial analysis (PubMed ID: 16382151): Normal phenotype: no abnormal phenotype detected: mice are fertile, viable and indistinguishable from wild-type mice. 2. Analysis of cultured primary hippocampal neurons (PubMed ID: 18321860): impairment of NMDA receptor-mediated signal transduction upon tPA stimulation. 3. Analysis after cross into LDLR-deficient background (PubMed ID: 19667105): enhanced postprandial dyslipidemia and atherosclerosis. 4. Functional analysis of immortalized MEFS (PubMed ID: 19667105 and 19856143): partial impairment of endocytic uptake of ligands. 5. Impact on plasma ApoM levels (PubMed ID: 20360257): elevated plasma ApoM levels. |
| Breeding history | Backcrossed 8 times to C57BL/6, only heterozygous mice available. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
Orphanet associated rare diseases, based on orthologous gene matching
