B6;129P2-Sox9^tm1Gsr/H

Status	Available to order
EMMA ID	EM:05334
Citation information	RRID:IMSR_EM:05334 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129P2-Sox9^tm1Gsr/H
Alternative name	Sox9-flox
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Sox9^tm1Gsr
Gene/Transgene symbol	Sox9

Information from provider

Provider	Ralf Kist
Provider affiliation	Institute of Human Genetics, Newcastle University
Genetic information	A region containing exons 2 and 3 was left floxed (flanked by loxP sites) after a floxed neo cassette was excised by the in vitro expression of cre recombinase.
Phenotypic information	Heterozygous and homozygous Sox9-flox mice are viable, fertile and appear normal. They can be used to conditionally inactivate Sox9 by crossing with cre recombinase-expressing mice.
Breeding history	Heterozygous Sox9-flox mice were backcrossed to C57BL/6 for three generations. Heterozygotes were then intercrossed to generate homozygous Sox9-flox mice which were subsequently maintained by sister-brother matings. The mice were backcrossed to C57BL/6J once after shipment to the MRC-Harwell.
References	Conditional inactivation of Sox9: a mouse model for campomelic dysplasia.;Kist Ralf, Schrewe Heinrich, Balling Rudi, Scherer Gerd, ;2002;Genesis (New York, N.Y. : 2000);32;121-3; 11857796 Homozygous inactivation of Sox9 causes complete XY sex reversal in mice.;Barrionuevo Francisco, Bagheri-Fam Stefan, Klattig Jürgen, Kist Ralf, Taketo Makoto M, Englert Christoph, Scherer Gerd, ;2006;Biology of reproduction;74;195-201; 16207837
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6J males
Breeding at archiving centre	Homozygous Sox9-flox mice were imported and backcrossed to C57BL/6J for one generation prior to archiving.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Campomelic dysplasia / Orphanet_140
46,XX ovotesticular disorder of sex development / Orphanet_2138
46,XX testicular disorder of sex development / Orphanet_393
Isolated Pierre Robin syndrome / Orphanet_718
46,XY complete gonadal dysgenesis / Orphanet_242
46,XY partial gonadal dysgenesis / Orphanet_251510

MGI phenotypes (gene matching)

short mandible / MGI
short maxilla / MGI
abnormal scapula morphology / MGI
abnormal sternum morphology / MGI
abnormal xiphoid process morphology / MGI
abnormal cartilage development / MGI
shortened head / MGI
domed cranium / MGI
short snout / MGI
decreased body weight / MGI
respiratory distress / MGI
abnormal tail morphology / MGI
abnormal skeleton development / MGI
no abnormal phenotype detected / MGI
abnormal primary sex determination / MGI
abnormal laryngeal cartilage morphology / MGI
micrognathia / MGI
abnormal bone mineralization / MGI
no phenotypic analysis / MGI
abnormal hyoid bone morphology / MGI
abnormal tracheal cartilage morphology / MGI
increased width of hypertrophic chondrocyte zone / MGI
abnormal long bone morphology / MGI
short sternum / MGI
abnormal sternebra morphology / MGI
small scapula / MGI
scapular bone hypoplasia / MGI
abnormal scapular spine morphology / MGI
absent deltoid tuberosity / MGI
bowed tibia / MGI
bowed ulna / MGI
bowed radius / MGI
abnormal pubis morphology / MGI
abnormal ischium morphology / MGI
small pubis / MGI
abnormal humerus morphology / MGI
abnormal ilium morphology / MGI
hearing/vestibular/ear phenotype / MGI
endocrine/exocrine gland phenotype / MGI
digestive/alimentary phenotype / MGI
reproductive system phenotype / MGI
vision/eye phenotype / MGI
decreased percent body fat/body weight / MGI
pancreatic islet hyperplasia / MGI
abnormal Meckel's cartilage morphology / MGI
congestive heart failure / MGI
decreased pancreatic beta cell mass / MGI
decreased pancreatic delta cell number / MGI
decreased PP cell number / MGI
meteorism / MGI
cleft secondary palate / MGI
bifurcated tongue / MGI
decreased total body fat amount / MGI
small thoracic cage / MGI
mortality/aging / MGI
neonatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI
abnormal head shape / MGI
decreased cranium length / MGI
embryonic lethality prior to organogenesis / MGI
decreased pancreatic alpha cell mass / MGI

Literature references

Conditional inactivation of Sox9: a mouse model for campomelic dysplasia.;Kist Ralf, Schrewe Heinrich, Balling Rudi, Scherer Gerd, ;2002;Genesis (New York, N.Y. : 2000);32;121-3; 11857796
Homozygous inactivation of Sox9 causes complete XY sex reversal in mice.;Barrionuevo Francisco, Bagheri-Fam Stefan, Klattig Jürgen, Kist Ralf, Taketo Makoto M, Englert Christoph, Scherer Gerd, ;2006;Biology of reproduction;74;195-201; 16207837
Fgf10 and Sox9 are essential for the establishment of distal progenitor cells during mouse salivary gland development.;Chatzeli Lemonia, Gaete Marcia, Tucker Abigail S, ;2017;Development (Cambridge, England);144;2294-2305; 28506998
Precancerous niche remodelling dictates nascent tumour persistence.;Skrupskelyte G, Rojo Arias J E, Ajith H, Dang Y, Rossetti D, Han S, Tang M K S, Bejar M T, Colom B, Fowler J C, Murai K, Knight W, Aust D, Schmidt M H H, Jászai J, Zeki S, Noorani A, Jones P H, Rulands S, Simons B D, Alcolea M P, ;2026;Nature;653;242-253; 41781610

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.

B6;129P2-Sox9tm1Gsr/H