C57BL/6-Tg(UBC-GFP,-Fkbp11)LPsou/Orl
| Status | Available to order |
| EMMA ID | EM:05353 |
| Citation information | RRID:IMSR_EM:05353 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(UBC-GFP,-Fkbp11)LPsou/Orl |
| Alternative name | FKBP11 LOW |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(UBC-GFP,-Fkbp11)LPsou |
| Gene/Transgene symbol | Tg(UBC-GFP,-Fkbp11)LPsou |
Information from provider
| Provider | Pauline Soulas-Sprauel |
| Provider affiliation | CRBS (Centre de Recherche en Biomédecine), Inserm UMR 1109 (Immuno-Rhumatologie Moléculaire, IRM) |
| Genetic information | FKBP11 LOW lentiviral transgenic mice have one copy of lentiviral integration with GFP reporter gene and display coexpression of GFP and FKBP11 thanks to a T2A sequence between GFP and FKBP11 sequences. |
| Phenotypic information | Normal development of mice. Increased number of follicular B cells in spleen. FKBP11 is overexpressed (qRT-PCR data) 9 times in bone marrow, 6 times in spleen, 6 times in sorted splenic B cells. However, GFP is not detectable by flow cytometry. |
| Breeding history | The transgenic strain was produced on C57BL/6 background, and maintained on C57BL/6 background by backcrosses. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
Literature references
- Overexpression of Fkbp11, a feature of lupus B cells, leads to B cell tolerance breakdown and initiates plasma cell differentiation.;Ruer-Laventie Julie, Simoni Léa, Schickel Jean-Nicolas, Soley Anne, Duval Monique, Knapp Anne-Marie, Marcellin Luc, Lamon Delphine, Korganow Anne-Sophie, Martin Thierry, Pasquali Jean-Louis, Soulas-Sprauel Pauline, ;2015;Immunity, inflammation and disease;3;265-79; 26417441