C57BL/6N-Tnfsf13btm1.1Arte/Ieg

Status

Available to order

EMMA IDEM:05501
Citation informationRRID:IMSR_EM:05501 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

 
International strain nameC57BL/6N-Tnfsf13btm1.1Arte/Ieg
Alternative nameTNFSF13b KO cond
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolTnfsf13btm1.1Arte
Gene/Transgene symbolTnfsf13b

Information from provider

Provider Helmholtz Zentrum Muenchen
Provider affiliationInstitute of Experimental Genetics, Helmholtz Zentrum Muenchen German Research Center for Environmental Health (GmbH)
Genetic informationThe mouse Tnfsf13b ORF is encoded by exons 1-7, with the TNF domain encoded by exons 4-7, while mouse exon 3 is not present in the human orthologue. Exons 5-6 have been flanked by loxP sites, as their genetic ablation should result in loss of function by deletion of more than 60% of the TNF domain. The conditional targeted mutation has been obtained by in vivo, flp recombinase-mediated removal of the neomycin selection marker.
Phenotypic informationTO BE PROVIDED
Breeding historyTargeting performed in C57BL/6N ES cells and confirmed by Southern blotting. Crossed to C57BL/6N-Tg(CAG-flpe)2Arte mice to obtain mutant founders with excised neo cassette. Maintained on C57BL/6N background.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requirednot known
Immunocompromisednot known

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6NTac males, wild-type C57BL/6N females
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (gene matching)
  • spleen hypoplasia / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • abnormal B cell differentiation / MGI
  • abnormal lymph organ size / MGI
  • abnormal lymph node B cell domain morphology / MGI
  • abnormal B cell physiology / MGI
  • decreased immunoglobulin level / MGI
  • increased length of allograft survival / MGI
  • decreased spleen weight / MGI
  • increased T cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell proliferation / MGI
  • decreased memory T cell number / MGI
  • increased single-positive T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased follicular B cell number / MGI
  • decreased marginal zone B cell number / MGI
  • decreased transitional stage B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • abnormal leukocyte morphology / MGI
  • impaired humoral immune response / MGI

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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