| Status | Available to order |
| EMMA ID | EM:05739 |
| Citation information | RRID:IMSR_EM:05739 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129S2(Cg)-Pax3tm3.1(Pax7)Buck/Orl |
| Alternative name | B6.Pax3Pax7ILZ/+ |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Pax3tm3.1(Pax7)Buck |
| Gene/Transgene symbol | Pax3 |
| Provider | Frederic RELAIX |
| Provider affiliation | Groupe Myologie, UMR-S 787 - INSERM - UPMC-Paris VI - Institut de Myologie |
| Additional owner | Margaret Buckingham, Molecular Genetics of Development (URA CNRS 2578), Institut Pasteur, Paris, France |
| Genetic information | The Pax7 gene followed by the IRESnLacZ reporters has been introduced in the Pax3 locus. To generate this line, floxed cassettes have been removed: one LoxP site remains in the genome. FLP-mediated recombination can remove the FRT flanked IRESnLacZ cassette inserted in the intron, leaving a FRT sequence. |
| Phenotypic information | The Pax3Pax7-ILZ/+ adult mice are viable and fertile and do not present the pigmentation phenotype of Pax3 heterozygous mice. Homozygotes are not viable: homozygous embryo die at birth. Homozygous loss-of-function mutation results limb muscle defects. Pax7 can substitute for Pax3 function in dorsal neural tube, neural crest cell, and somite development, but not in the formation of muscles involving long-range migration of muscle progenitor cells. The double homozygotes are not viable: homozygous embryo die at birth. |
| Breeding history | Heterozygotes are viable and fertile. Homozygotes are not viable. Heterozygous males are crossed to C57BL/6 females. The line has been backcrossed to C57BL/6 background for at least 10 generations. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |
Orphanet associated rare diseases, based on orthologous gene matching
