B6.Cg-Trerf1tm1.1Pas/Orl
| Status | Available to order |
| EMMA ID | EM:05761 |
| Citation information | RRID:IMSR_EM:05761 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Trerf1tm1.1Pas/Orl |
| Alternative name | TReP-132 floxed |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Trerf1tm1.1Pas |
| Gene/Transgene symbol | Trerf1 |
Information from provider
| Provider | Barbara Gross |
| Provider affiliation | Unité animale et laboratoire de haute sécurité, Institut Pasteur de Lille |
| Genetic information | Two loxP sites were introduced in intron 1 and intron 4 of the TReP-132 (Trerf1) gene by gene targeting in ES cells. The neomycin expression cassette has been removed using the FRT/Flp system, therefore a FRT site is also present in the vicinity of the loxP site in intron 4. Conditional excision with a cre recombinase will remove the region encompassing exon 2 to exon 4 that encodes for the start codon, the first zinc finger and the first LXXLL motif. |
| Phenotypic information | The mouse is "floxed" for TReP-132 (Trerf1) - as such, gene expression of TReP-132 is not modified. Therefore it behaves as a wild type mouse. Phenotype can be induced by crossing the mouse with a cre recombinase-expressing transgenic mouse. Human TReP-132 was shown to be involved in the regulation of steroidogenesis and of the cell cycle. |
| Breeding history | 10th generation of backcross to C57BL/6J. Currently bred as an homozygous colony. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous C57BL/6J males, wild-type C57BL/6J females |
Literature references
- TReP-132 is a novel progesterone receptor coactivator required for the inhibition of breast cancer cell growth and enhancement of differentiation by progesterone.;Gizard Florence, Robillard Romain, Gross Barbara, Barbier Olivier, Révillion Françoise, Peyrat Jean-Philippe, Torpier Gérard, Hum Dean W, Staels Bart, ;2006;Molecular and cellular biology;26;7632-44; 17015480
- TReP-132 controls cell proliferation by regulating the expression of the cyclin-dependent kinase inhibitors p21WAF1/Cip1 and p27Kip1.;Gizard Florence, Robillard Romain, Barbier Olivier, Quatannens Brigitte, Faucompré Anne, Révillion Françoise, Peyrat Jean-Philippe, Staels Bart, Hum Dean W, ;2005;Molecular and cellular biology;25;4335-48; 15899840
- The transcriptional regulating protein of 132 kDa (TReP-132) differentially influences steroidogenic pathways in human adrenal NCI-H295 cells.;Gizard F, Teissier E, Dufort I, Luc G, Luu-The V, Staels B, Hum D W, ;2004;Journal of molecular endocrinology;32;557-69; 15072559
- The transcriptional regulating protein of 132 kDa (TReP-132) enhances P450scc gene transcription through interaction with steroidogenic factor-1 in human adrenal cells.;Gizard Florence, Lavallee Bernard, DeWitte Frederique, Teissier Elisabeth, Staels Bart, Hum Dean W, ;2002;The Journal of biological chemistry;277;39144-55; 12101186
- Cloning of murine TReP-132, a novel transcription factor expressed in brain regions involved in behavioral and psychiatric disorders.;Duguay Y, Lapointe A, Lavallée B, Hum D W, Rivest S, ;2003;Molecular psychiatry;8;39-49; 12556907
- A novel zinc finger protein TReP-132 interacts with CBP/p300 to regulate human CYP11A1 gene expression.;Gizard F, Lavallée B, DeWitte F, Hum D W, ;2001;The Journal of biological chemistry;276;33881-92; 11349124