B6.129P2-Txnrd1^tm1Marc/Ieg

Status	Available to order
EMMA ID	EM:05844
Citation information	RRID:IMSR_EM:05844 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129P2-Txnrd1^tm1Marc/Ieg
Alternative name	Txnrd1tm1Marc (thioredoxin reductase 1)
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Txnrd1^tm1Marc
Gene/Transgene symbol	Txnrd1

Information from provider

Provider	Marcus Conrad
Provider affiliation	Institut of Clinical Molecular Biology and Tumor Genetics, Helmholtz Zentrum Muenchen
Genetic information	Exon 15, which encodes 22 amino acids and the C-terminally located redox center consisting of Gly-Cys-Sec-Gly of Txnrd1, and 1.7 kb of the 3' UTR containing the selenocysteine insertion element, essential for cotranslational Sec incorporation at the UGA codon, AU-rich mRNA instability elements and the endogenous transcription stop signal, were flanked by loxP sites. Cre-mediated removal of the last exon leads to a dysfunctional Txnrd1 allele.
Phenotypic information	Ubiquitous Cre-mediated removal of both Txnrd1 causes early embryonic death between E8.5 and E9.5 due to severe overall growth and developmental retardation. Heart-specific Txnrd1 deletion does not cause any overt impairment of cardiac development and function, whereas central-nervous system specific Txnrd1 inactivation causes massive cerebellar hypoplasia and ataxia.
Breeding history	More than 10 generations backcrossed on C57BL/6.
References	Cytoplasmic thioredoxin reductase is essential for embryogenesis but dispensable for cardiac development.;Jakupoglu Cemile, Przemeck Gerhard K H, Schneider Manuela, Moreno Stéphanie G, Mayr Nadja, Hatzopoulos Antonis K, de Angelis Martin Hrabé, Wurst Wolfgang, Bornkamm Georg W, Brielmeier Markus, Conrad Marcus, ;2005;Molecular and cellular biology;25;1980-8; 15713651 The role of thioredoxin reductases in brain development.;Soerensen Jonna, Jakupoglu Cemile, Beck Heike, Förster Heidi, Schmidt Jörg, Schmahl Wolfgang, Schweizer Ulrich, Conrad Marcus, Brielmeier Markus, ;2008;PloS one;3;e1813; 18350150 Mitochondrial thioredoxin reductase is essential for early postischemic myocardial protection.;Horstkotte Jan, Perisic Tamara, Schneider Manuela, Lange Philipp, Schroeder Melanie, Kiermayer Claudia, Hinkel Rabea, Ziegler Tilman, Mandal Pankaj K, David Robert, Schulz Sabine, Schmitt Sabine, Widder Julian, Sinowatz Fred, Becker Bernhard F, Bauersachs Johann, Naebauer Michael, Franz Wolfgang M, Jeremias Irmela, Brielmeier Markus, Zischka Hans, Conrad Marcus, Kupatt Christian, ;2011;Circulation;124;2892-902; 22144571 System x(c)- and thioredoxin reductase 1 cooperatively rescue glutathione deficiency.;Mandal Pankaj Kumar, Seiler Alexander, Perisic Tamara, Kölle Pirkko, Banjac Canak Ana, Förster Heidi, Weiss Norbert, Kremmer Elisabeth, Lieberman Michael W, Bannai Shiro, Kuhlencordt Peter, Sato Hideyo, Bornkamm Georg W, Conrad Marcus, ;2010;The Journal of biological chemistry;285;22244-53; 20463017 Loss of thioredoxin reductase 1 renders tumors highly susceptible to pharmacologic glutathione deprivation.;Mandal Pankaj Kumar, Schneider Manuela, Kölle Pirkko, Kuhlencordt Peter, Förster Heidi, Beck Heike, Bornkamm Georg W, Conrad Marcus, ;2010;Cancer research;70;9505-14; 21045148
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI phenotypes (gene matching)

decreased cell proliferation / MGI
failure of primitive streak formation / MGI
failure to gastrulate / MGI
decreased embryo size / MGI
abnormal visceral yolk sac morphology / MGI
embryonic growth arrest / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
failure of initiation of embryo turning / MGI
increased trophoblast giant cell number / MGI
impaired somite development / MGI
embryonic lethality between somite formation and embryo turning, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
absent embryonic epiblast / MGI
abnormal visceral endoderm morphology / MGI
absent fibroblast proliferation / MGI
short rostral-caudal axis / MGI

Literature references

Cytoplasmic thioredoxin reductase is essential for embryogenesis but dispensable for cardiac development.;Jakupoglu Cemile, Przemeck Gerhard K H, Schneider Manuela, Moreno Stéphanie G, Mayr Nadja, Hatzopoulos Antonis K, de Angelis Martin Hrabé, Wurst Wolfgang, Bornkamm Georg W, Brielmeier Markus, Conrad Marcus, ;2005;Molecular and cellular biology;25;1980-8; 15713651
The role of thioredoxin reductases in brain development.;Soerensen Jonna, Jakupoglu Cemile, Beck Heike, Förster Heidi, Schmidt Jörg, Schmahl Wolfgang, Schweizer Ulrich, Conrad Marcus, Brielmeier Markus, ;2008;PloS one;3;e1813; 18350150
Mitochondrial thioredoxin reductase is essential for early postischemic myocardial protection.;Horstkotte Jan, Perisic Tamara, Schneider Manuela, Lange Philipp, Schroeder Melanie, Kiermayer Claudia, Hinkel Rabea, Ziegler Tilman, Mandal Pankaj K, David Robert, Schulz Sabine, Schmitt Sabine, Widder Julian, Sinowatz Fred, Becker Bernhard F, Bauersachs Johann, Naebauer Michael, Franz Wolfgang M, Jeremias Irmela, Brielmeier Markus, Zischka Hans, Conrad Marcus, Kupatt Christian, ;2011;Circulation;124;2892-902; 22144571
System x(c)- and thioredoxin reductase 1 cooperatively rescue glutathione deficiency.;Mandal Pankaj Kumar, Seiler Alexander, Perisic Tamara, Kölle Pirkko, Banjac Canak Ana, Förster Heidi, Weiss Norbert, Kremmer Elisabeth, Lieberman Michael W, Bannai Shiro, Kuhlencordt Peter, Sato Hideyo, Bornkamm Georg W, Conrad Marcus, ;2010;The Journal of biological chemistry;285;22244-53; 20463017
Loss of thioredoxin reductase 1 renders tumors highly susceptible to pharmacologic glutathione deprivation.;Mandal Pankaj Kumar, Schneider Manuela, Kölle Pirkko, Kuhlencordt Peter, Förster Heidi, Beck Heike, Bornkamm Georg W, Conrad Marcus, ;2010;Cancer research;70;9505-14; 21045148

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B6.129P2-Txnrd1tm1Marc/Ieg