C57BL/6N-Tg(CRP-Cast)1Lbau/Orl
| Status | Available to order |
| EMMA ID | EM:05917 |
| Citation information | RRID:IMSR_EM:05917 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6N-Tg(CRP-Cast)1Lbau/Orl |
| Alternative name | CRP/Calpast |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CRP-Cast)1Lbau |
| Gene/Transgene symbol | Tg(CRP-Cast)1Lbau |
Information from provider
| Provider | Laurent BAUD |
| Provider affiliation | UMR_S 702, INSERM / UPMC |
| Genetic information | Calpains are calcium-activated neutral cysteine proteases. Although calpains were first described as intracellular enzymes, more recent studies show that they are partly externalized. To prove strictly that calpains in the extracellular milieu have a pathophysiological role, we have chosen to develop a novel transgenic mouse with inflammation-inducible expression of secreted calpastatin to blunt specifically and solely extracellular calpains. |
| Phenotypic information | No phenotype in the absence of inflammatory stress. |
| Breeding history | Two different transgenic founders have been obtained. Each founder has been crossed with wild type mice. Thereafter, male and female pups exhibiting the transgene have been crossed to obtain the third generation of mice. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6N males, wild-type C57BL/6N females |
| Stage of embryos | 2-cell |
Literature references
- Transgenic mice showing inflammation-inducible overexpression of granulocyte macrophage colony-stimulating factor.;Burke B, Pridmore A, Harraghy N, Collick A, Brown J, Mitchell T, ;2004;Clinical and diagnostic laboratory immunology;11;588-98; 15138187
- Calpains Released by T Lymphocytes Cleave TLR2 To Control IL-17 Expression.;Perez Joëlle, Dansou Boris, Hervé Roxane, Levi Charlène, Tamouza Houda, Vandermeersch Sophie, Demey-Thomas Emmanuelle, Haymann Jean-Philippe, Zafrani Lara, Klatzmann David, Boissier Marie-Christophe, Letavernier Emmanuel, Baud Laurent, ;2016;Journal of immunology (Baltimore, Md. : 1950);196;168-81; 26608921
- Cleavage of IκBα by calpain induces myocardial NF-κB activation, TNF-α expression, and cardiac dysfunction in septic mice.;Li Xiaoping, Luo Rong, Chen Ruizhen, Song Li, Zhang Shu, Hua Wei, Chen Haozhu, ;2014;American journal of physiology. Heart and circulatory physiology;306;H833-43; 24441549
- Targeted inhibition of calpain reduces myocardial hypertrophy and fibrosis in mouse models of type 1 diabetes.;Li Ying, Ma Jian, Zhu Huaqing, Singh Manpreet, Hill David, Greer Peter A, Arnold J Malcolm, Abel E Dale, Peng Tianqing, ;2011;Diabetes;60;2985-94; 21911754
- Preventing Calpain Externalization by Reducing ABCA1 Activity with Probenecid Limits Melanoma Angiogenesis and Development.;Hanouna Guillaume, Tang Ellie, Perez Joëlle, Vandermeersch Sophie, Haymann Jean-Philippe, Baud Laurent, Letavernier Emmanuel, ;2020;The Journal of investigative dermatology;140;445-454; 31425704