IMPC phenotypes (gene matching)
129S2/SvPas-Htr2btm1Lum/Orl
| Status | Available to order |
| EMMA ID | EM:05936 |
| Citation information | RRID:IMSR_EM:05936 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129S2/SvPas-Htr2btm1Lum/Orl |
| Alternative name | 5-HT2B Ex3 =ICS UQ54-13= JAX Htr2btm1Lum in 129S2 background |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Htr2btm1Lum |
| Gene/Transgene symbol | Htr2b |
Information from provider
| Provider | Luc Maroteaux |
| Provider affiliation | Institut du Fer a Moulin, INSERM UMR S-839 |
| Genetic information | Mice with neomycin cassette inserted into the 3rd exon (2nd coding exon) of Htr2b gene, coding for the 5-HT2B (serotonin) receptor, by homologous recombination inducing permanent inhibition of receptor expression. |
| Phenotypic information | The 5-HT2B-/- mice were reported (Nebigil et al, 2000) to display midgestation and postnatal partial lethality due to cardiac defects. The 5-HT2B receptor-mutant mice that survived the first postnatal week (about 50%) developed to adulthood with cardiac problems, though these become compensated with age (Jaffre et al, 2009) and 10-20 weeks old mice have no more detectable defects. The 5-HT2B receptor-mutant mice display no basal pulmonary phenotype, instead they are resistant to inducers of pulmonary hypertension (Launay et al, 2002). So the cardiovascular and pulmonary phenotypes are undetectable in living adult mice. |
| Breeding history | The targeting vector was constructed with the selective bacterial neo gene introduced into the exon 2 sequence of the 5-HT2B receptor locus. This resulted in a non-functional allele of 5-HT2B as it interrupts the protein reading frame. One recombination event was obtained after screening of about 4000 ES cell clones. Southern blot analysis on the recombined clone using a probe derived from neo sequence revealed that it contained a single expected 16 Kbp fragment in an HpaI restriction enzyme digestion excluding multiple insertion of the targeting vector. From this recombined 5-HT2B ES cell strain, 13 chimeric animals were created by injection into C57BL/6 blastocyst embryos. These chimeras were bred to either C57BL/6NCrl or 129S2/SvPas mouse strain to obtain germ-line transmission. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | homozygous 129S2/SvPas males, wild-type 129S2/SvPas females |
| Breeding at archiving centre | Some wild-type females provided by the company potentially have a mixed background: B6.CBAx129/SvPas. It is possible that some frozen embryos have the same background. |
| Stage of embryos | 8-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- abnormal heart development / MGI
- absent trabeculae carneae / MGI
- decreased body weight / MGI
- abnormal cardiovascular system physiology / MGI
- decreased embryo size / MGI
- reduced fertility / MGI
- abnormal cardiovascular system morphology / MGI
- disorganized myocardium / MGI
- decreased heart weight / MGI
- dilated heart atrium / MGI
- abnormal fetal cardiomyocyte proliferation / MGI
- embryonic growth retardation / MGI
- abnormal heart ventricle morphology / MGI
- cardiovascular system phenotype / MGI
- decreased right ventricle systolic pressure / MGI
- postnatal lethality, incomplete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
MGI phenotypes (gene matching)
- abnormal heart development / MGI
- absent trabeculae carneae / MGI
- decreased body weight / MGI
- abnormal cardiovascular system physiology / MGI
- decreased embryo size / MGI
- reduced fertility / MGI
- abnormal cardiovascular system morphology / MGI
- no abnormal phenotype detected / MGI
- disorganized myocardium / MGI
- decreased heart weight / MGI
- dilated heart atrium / MGI
- abnormal fetal cardiomyocyte proliferation / MGI
- embryonic growth retardation / MGI
- abnormal heart ventricle morphology / MGI
- cardiovascular system phenotype / MGI
- decreased right ventricle systolic pressure / MGI
- postnatal lethality, incomplete penetrance / MGI
- embryonic lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
Literature references
- Serotonin 2B receptor is required for heart development.;Nebigil C G, Choi D S, Dierich A, Hickel P, Le Meur M, Messaddeq N, Launay J M, Maroteaux L, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;9508-13; 10944220
- Ablation of serotonin 5-HT(2B) receptors in mice leads to abnormal cardiac structure and function.;Nebigil C G, Hickel P, Messaddeq N, Vonesch J L, Douchet M P, Monassier L, György K, Matz R, Andriantsitohaina R, Manivet P, Launay J M, Maroteaux L, ;2001;Circulation;103;2973-9; 11413089
- Function of the serotonin 5-hydroxytryptamine 2B receptor in pulmonary hypertension.;Launay J-M, Hervé P, Peoc'h K, Tournois C, Callebert J, Nebigil C G, Etienne N, Drouet L, Humbert M, Simonneau G, Maroteaux L, ;2002;Nature medicine;8;1129-35; 12244304
- Serotonin is a novel survival factor of cardiomyocytes: mitochondria as a target of 5-HT2B receptor signaling.;Nebigil Canan G, Etienne Nelly, Messaddeq Nadia, Maroteaux Luc, ;2003;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;17;1373-5; 12738797
- Involvement of the serotonin 5-HT2B receptor in cardiac hypertrophy linked to sympathetic stimulation: control of interleukin-6, interleukin-1beta, and tumor necrosis factor-alpha cytokine production by ventricular fibroblasts.;Jaffré Fabrice, Callebert Jacques, Sarre Alexandre, Etienne Nelly, Nebigil Canan G, Launay Jean-Marie, Maroteaux Luc, Monassier Laurent, ;2004;Circulation;110;969-74; 15302781
- Endogenous 5-HT2B receptor activation regulates neonatal respiratory activity in vitro.;Günther Silke, Maroteaux Luc, Schwarzacher Stephan W, ;2006;Journal of neurobiology;66;949-61; 16758492
- The serotonin 5-HT2B receptor controls bone mass via osteoblast recruitment and proliferation.;Collet C, Schiltz C, Geoffroy V, Maroteaux L, Launay J-M, de Vernejoul M-C, ;2008;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;22;418-27; 17846081
- Serotonin 5-HT2B receptors are required for 3,4-methylenedioxymethamphetamine-induced hyperlocomotion and 5-HT release in vivo and in vitro.;Doly Stéphane, Valjent Emmanuel, Setola Vincent, Callebert Jacques, Hervé Denis, Launay Jean-Marie, Maroteaux Luc, ;2008;The Journal of neuroscience : the official journal of the Society for Neuroscience;28;2933-40; 18337424
- Serotonin and angiotensin receptors in cardiac fibroblasts coregulate adrenergic-dependent cardiac hypertrophy.;Jaffré Fabrice, Bonnin Philippe, Callebert Jacques, Debbabi Haythem, Setola Vincent, Doly Stéphane, Monassier Laurent, Mettauer Bertrand, Blaxall Burns C, Launay Jean-Marie, Maroteaux Luc, ;2009;Circulation research;104;113-23; 19023134
- Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo.;Tharayil V S, Wouters M M, Stanich J E, Roeder J L, Lei S, Beyder A, Gomez-Pinilla P J, Gershon M D, Maroteaux L, Gibbons S J, Farrugia G, ;2010;Neurogastroenterology and motility;22;462-9, e109-10; 19941613
- Deconstructing antiobesity compound action: requirement of serotonin 5-HT2B receptors for dexfenfluramine anorectic effects.;Banas Sophie M, Doly Stéphane, Boutourlinsky Katia, Diaz Silvina L, Belmer Arnauld, Callebert Jacques, Collet Corinne, Launay Jean-Marie, Maroteaux Luc, ;2011;Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology;36;423-33; 20927048
- A population-specific HTR2B stop codon predisposes to severe impulsivity.;Bevilacqua Laura, Doly Stéphane, Kaprio Jaakko, Yuan Qiaoping, Tikkanen Roope, Paunio Tiina, Zhou Zhifeng, Wedenoja Juho, Maroteaux Luc, Diaz Silvina, Belmer Arnaud, Hodgkinson Colin A, Dell'osso Liliana, Suvisaari Jaana, Coccaro Emil, Rose Richard J, Peltonen Leena, Virkkunen Matti, Goldman David, ;2010;Nature;468;1061-6; 21179162
- Platelet-derived serotonin links vascular disease and tissue fibrosis.;Dees Clara, Akhmetshina Alfiya, Zerr Pawel, Reich Nicole, Palumbo Katrin, Horn Angelika, Jüngel Astrid, Beyer Christian, Krönke Gerhard, Zwerina Jochen, Reiter Rudolf, Alenina Natalia, Maroteaux Luc, Gay Steffen, Schett Georg, Distler Oliver, Distler Jörg H W, ;2011;The Journal of experimental medicine;208;961-72; 21518801
- Stimulating healthy tissue regeneration by targeting the 5-HT₂B receptor in chronic liver disease.;Ebrahimkhani Mohammad R, Oakley Fiona, Murphy Lindsay B, Mann Jelena, Moles Anna, Perugorria Maria J, Ellis Elizabeth, Lakey Anne F, Burt Alastair D, Douglass Angela, Wright Matthew C, White Steven A, Jaffré Fabrice, Maroteaux Luc, Mann Derek A, ;2011;Nature medicine;17;1668-73; 22120177
- 5-HT(2B) receptors are required for serotonin-selective antidepressant actions.;Diaz S L, Doly S, Narboux-Nême N, Fernández S, Mazot P, Banas S M, Boutourlinsky K, Moutkine I, Belmer A, Roumier A, Maroteaux L, ;2012;Molecular psychiatry;17;154-63; 22158014
- Implication of 5-HT(2B) receptors in the serotonin syndrome.;Diaz Silvina Laura, Maroteaux Luc, ;2011;Neuropharmacology;61;495-502; 21277875
- Serotonin 5-HT2B receptors are required for bone-marrow contribution to pulmonary arterial hypertension.;Launay Jean-Marie, Hervé Philippe, Callebert Jacques, Mallat Ziad, Collet Corinne, Doly Stéphane, Belmer Arnauld, Diaz Silvina L, Hatia Sarah, Côté Francine, Humbert Marc, Maroteaux Luc, ;2012;Blood;119;1772-80; 22186990