IMPC phenotypes (gene matching)
129(B6)-Htr2btm2Lum/Ics
| Status | Available to order |
| EMMA ID | EM:05939 |
| Citation information | RRID:IMSR_EM:05939 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | 129(B6)-Htr2btm2Lum/Ics |
| Alternative name | 5-HT2B Ex2 flx, ICS K413 L3 (clone 266 -projet IR1446) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Htr2btm2Lum |
| Gene/Transgene symbol | Htr2b |
Information from provider
| Provider | Luc Maroteaux |
| Provider affiliation | Institut du Fer a Moulin, INSERM UMR S-839 |
| Genetic information | For the gene targeting construct, a 10 kb BamHI-XhoI fragment containing the two first Htr2b exons was selected, while a 4 kb SacI-SacI fragment containing exon 2, which includes the ATG start codon and 5' UTR was used to induce the targeted deletion. A loxP site was inserted in the 5' UTR (2nd exon) and a neomycin-resistance cassette flanked by two loxP sites was inserted in the ClaI site of the second intron. The SacI-SacI fragment comprising the floxed construct was then excised and electroporated into 129S2 embryonic stem cells which were subjected to G418 selection. Targeted homologous recombination was confirmed by PCR and Southern blot analysis. A positive ES clone was injected into C57BL/6NCrl blastocysts and implanted into pseudopregnant mice. A chimeric male displaying germline transmission was then used to propagate the floxed Htr2b allele on a C57BL/6NCrl background for the first two generations. Seven backcrosses of HTR2B/flox mice to 129S2 wild-type mice were performed. |
| Phenotypic information | This mutant strain with floxed Htr2b locus allows the generation of mice with locally inactivated Htr2b, using either crosses with mice expressing cre recombinase in selected areas of interest or by local injection of virally encoded cre recombinase. |
| Breeding history | A positive 129S2 ES clone was injected into C57BL/6NCrl blastocysts and implanted into pseudopregnant mice. A chimeric male displaying germline transmission was then used to propagate the floxed Htr2b allele on a C57BL/6NCrl background for the two first generations. Seven backcrosses of HTR2B/flox mice to 129S2 wild-type mice were performed. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
| Animals used for archiving | heterozygous C57BL/6;129 males, wild-type C57BL/6NTac females |
| Stage of embryos | 8-cell |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal heart development / MGI
- absent trabeculae carneae / MGI
- decreased body weight / MGI
- abnormal cardiovascular system physiology / MGI
- decreased embryo size / MGI
- reduced fertility / MGI
- abnormal cardiovascular system morphology / MGI
- no abnormal phenotype detected / MGI
- disorganized myocardium / MGI
- decreased heart weight / MGI
- dilated heart atrium / MGI
- abnormal fetal cardiomyocyte proliferation / MGI
- embryonic growth retardation / MGI
- abnormal heart ventricle morphology / MGI
- cardiovascular system phenotype / MGI
- decreased right ventricle systolic pressure / MGI
- postnatal lethality, incomplete penetrance / MGI
- embryonic lethality, complete penetrance / MGI
- embryonic lethality during organogenesis, incomplete penetrance / MGI
Literature references
- Overexpression of the serotonin 5-HT2B receptor in heart leads to abnormal mitochondrial function and cardiac hypertrophy.;Nebigil Canan G, Jaffré Fabrice, Messaddeq Nadia, Hickel Pierre, Monassier Laurent, Launay Jean-Marie, Maroteaux Luc, ;2003;Circulation;107;3223-9; 12810613
- Serotonin sensing by microglia conditions the proper development of neuronal circuits and of social and adaptive skills.;Albertini Giulia, D'Andrea Ivana, Druart Mélanie, Béchade Catherine, Nieves-Rivera Nayadoleni, Etienne Fanny, Le Magueresse Corentin, Rebsam Alexandra, Heck Nicolas, Maroteaux Luc, Roumier Anne, ;2023;Molecular psychiatry;28;2328-2342; 37217677
- 5-HT1A and 5-HT2B receptor interaction and co-clustering regulate serotonergic neuron excitability.;Benhadda Amina, Delhaye Célia, Moutkine Imane, Marques Xavier, Russeau Marion, Le Magueresse Corentin, Roumier Anne, Lévi Sabine, Maroteaux Luc, ;2023;iScience;26;107401; 37575185