| Status | Available to order |
| EMMA ID | EM:05941 |
| Citation information | RRID:IMSR_EM:05941 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cdk2tm1Sgo/Cnbc |
| Alternative name | Cdk2 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cdk2tm1Sgo |
| Gene/Transgene symbol | Cdk2 |
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | A neomycin resistance gene flanked by loxP sites was originally inserted into intron 3 while a third loxP site was inserted into intron 1. Resulting mice were then crossed with mice carrying a CMV-cre transgene which resulted in the excision of the neomycin resistance gene and exons 2 and 3 of Cdk2 leading to a predicted reading frame shift. |
| Phenotypic information | Homozygous males and females are sterile due to the complete lack of germ cells. No other obvious phenotype is detected in homozygosis. Heterozygous animals are indistinguishable from WT. |
| Breeding history | Chimeras were crossed with CD1 females for germ line transmission analysis. Heterozygous offspring were crossed with Tg(CMV-cre)1Nagy for deleting the neomycin cassette and exons 2 and 3 of the Cdk2 gene. Line was maintained by inter-crossing heterozygous animals or by crossing heterozygous for Cdk2 with CD1 mice. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous mixed males, wild-type CD-1 females |
| Stage of embryos | 8-cell |
