B6;129-Fli1^tm1Morl/Orl

Status	Available to order
EMMA ID	EM:05991
Citation information	RRID:IMSR_EM:05991 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129-Fli1^tm1Morl/Orl
Alternative name	Fli1-fl/fl
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Fli1^tm1Morl
Gene/Transgene symbol	Fli1

Information from provider

Provider	Francois Morle
Provider affiliation	CGPhyMC , CNRS UMR5534
Genetic information	This strain carries two loxP sequences flanking the whole coding region of Fli1 gene exon 9 that have been inserted in intron 8 and 3' UTR respectively. The FRT flanked neo gene cassette initially inserted in 3' UTR and used to select homologous recombinant ES cells clones has been removed by transient FLP expression before generating the transgenic founder of this strain. This strain allows conditional Cre-mediated deletion of Fli1 gene exon 9 encoding the whole DNA-binding and C-terminal regions of Fli1 transcription factor.
Phenotypic information	Contrasting with lethality of Fli1 -/- genotype, homozygous Fli1 flox/flox mice are viable and fertile and display no obvious phenotype. Cre recombinase induction in homozygous Fli1 flox/flox Mx1-cre+ or heterozygous Fli1 flox/- Mx1-cre+ mice leads to multiple commitment decisions and proliferation/differentiation alterations in adult hematopoiesis.
Breeding history	LoxP insertions at Fli1 locus were generated in 129P2 ES cells. Cells were injected in C57BL/6J blastocysts and backcrossed on C57BL/6J.
References	Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.;Starck Joëlle, Weiss-Gayet Michèle, Gonnet Colette, Guyot Boris, Vicat Jean-Michel, Morlé François, ;2010;Blood;116;4795-805; 20733157
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France
Animals used for archiving	homozygous C57BL/6J males, wild-type C57BL/6J females
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Isolated delta-storage pool disease / Orphanet_248340

MGI phenotypes (gene matching)

decreased hematocrit / MGI
abnormal vascular development / MGI
small liver / MGI
pale liver / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
decreased thymocyte number / MGI
anemia / MGI
impaired hematopoiesis / MGI
decreased embryo size / MGI
pale yolk sac / MGI
abnormal humoral immune response / MGI
intracranial hemorrhage / MGI
intraventricular hemorrhage / MGI
increased leukemia incidence / MGI
abnormal B cell differentiation / MGI
abnormal megakaryocyte progenitor cell morphology / MGI
abnormal proerythroblast morphology / MGI
abnormal megakaryocyte morphology / MGI
abnormal class switch recombination / MGI
decreased B cell number / MGI
decreased B cell proliferation / MGI
spinal hemorrhage / MGI
decreased follicular B cell number / MGI
increased marginal zone B cell number / MGI
increased transitional stage B cell number / MGI
decreased megakaryocyte cell number / MGI
decreased IgG1 level / MGI
decreased IgG3 level / MGI
abnormal splenic cell ratio / MGI
abnormal brain meninges morphology / MGI
embryonic lethality during organogenesis, complete penetrance / MGI

Literature references

Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.;Starck Joëlle, Weiss-Gayet Michèle, Gonnet Colette, Guyot Boris, Vicat Jean-Michel, Morlé François, ;2010;Blood;116;4795-805; 20733157

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6;129-Fli1tm1Morl/Orl