B6.Cg-Tg(TG-KCNIP3*)15Cnbc/Cnbc

Status	Available to order
EMMA ID	EM:06009
Citation information	RRID:IMSR_EM:06009 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Tg(TG-KCNIP3*)15Cnbc/Cnbc
Alternative name	B6.B6CBA-Tg(bTg-hLeuEFmDREAM)LeuBTA15Cnbc
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(TG-KCNIP3*)15Cnbc
Gene/Transgene symbol	Tg(TG-KCNIP3*)15Cnbc

Information from provider

Provider	Jose Ramon Naranjo
Provider affiliation	Centro Nacional de Biotecnologia (CNB-CSIC)
Genetic information	The proximal bovine thyroglobulin (TG) promoter was used to target the dominant active KCNIP3 (DREAM) mutant insensitive to calcium and cAMP (LeuEFmDREAM) to the thyroid gland. The transgenic cassette was microinjected into the pronuclei of one-cell embryos (B6CBA) using standard techniques. Founder mice were backcrossed to C57BL/6 background.
Phenotypic information	High level of transgene expression. Thyroid glands from 9- to 12-months old mice showed a marked enlargement of the transgenic gland compared with wild-type, that could be either diffuse, resembling a colloid goiter, or nodular, resembling human multinodular goiters.
Breeding history	Generated in B6CBA background and backcrossed (>10 times) to C57BL/6. Currently maintained as hemizygous in C57BL/6 background.
References	The DREAM protein is associated with thyroid enlargement and nodular development.;Rivas Marcos, Mellström Britt, Torres Begoña, Cali Gaetano, Ferrara Alfonso M, Terracciano Daniela, Zannini Mariastella, Morreale de Escobar Gabriella, Naranjo Jose R, ;2009;Molecular endocrinology (Baltimore, Md.);23;862-70; 19299442
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous (C57BL/6 x CBA)F2 males, wild-type C57BL/6 females
Stage of embryos	8-cell

Literature references

The DREAM protein is associated with thyroid enlargement and nodular development.;Rivas Marcos, Mellström Britt, Torres Begoña, Cali Gaetano, Ferrara Alfonso M, Terracciano Daniela, Zannini Mariastella, Morreale de Escobar Gabriella, Naranjo Jose R, ;2009;Molecular endocrinology (Baltimore, Md.);23;862-70; 19299442

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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