IMPC phenotypes (gene matching)
C3;129S7-Hprt1tm2Brd/Biat
| Status | Available to order |
| EMMA ID | EM:06049 |
| Citation information | RRID:IMSR_EM:06049 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C3;129S7-Hprt1tm2Brd/Biat |
| Alternative name | HPRT1-Myo7a-EGFP-betaActin-SV40pA-BAC transgene |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Hprt1tm2Brd |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | Haydn Prosser |
| Provider affiliation | Wellcome Trust Sanger Institute |
| Genetic information | The transgene contains EGFP-beta-actin-SV40pA in place of the myosin 7a translation initiation codon within the BAC clone RPCI23-138G2. The modified BAC was site specifically integrated at the modified Hprt1 locus by cre recombinase-mediated cassette exchange (RMCE). |
| Phenotypic information | The mouse strain expresses EGFP-beta actin in mouse inner ear hair cells and can be used to fluorescently label stereocilia. In heterozygous females the labelling is mosaic. |
| Breeding history | The transgenic was created in AB1 derived 129 ES cells. Chimaeras produced from the ES cells were mated with C3H strain. Strain was then maintained by intercrossing F1 and subsequent generations without further backcrossing. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- abnormal small intestine morphology / MGI
- abnormal liver morphology / MGI
- abnormal branching of the mammary ductal tree / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- tremors / MGI
- convulsive seizures / MGI
- abnormal lung morphology / MGI
- decreased body weight / MGI
- decreased anxiety-related response / MGI
- ataxia / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- short stride length / MGI
- decreased exploration in new environment / MGI
- limb grasping / MGI
- abnormal motor coordination/balance / MGI
- abnormal hematopoietic system physiology / MGI
- hyperglycemia / MGI
- anemia / MGI
- cardiac hypertrophy / MGI
- increased mammary adenocarcinoma incidence / MGI
- abnormal reflex / MGI
- seizures / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal brain morphology / MGI
- no abnormal phenotype detected / MGI
- neurodegeneration / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- hepatic steatosis / MGI
- decreased vertical activity / MGI
- increased heart weight / MGI
- increased systemic arterial blood pressure / MGI
- albuminuria / MGI
- decreased erythrocyte cell number / MGI
- increased urine protein level / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- phenotypic reversion / MGI
- abnormal dopaminergic neuron morphology / MGI
- astrocytosis / MGI
- abnormal depression-related behavior / MGI
- decreased tumor growth/size / MGI
- abnormal nervous system morphology / MGI
- abnormal cardiac muscle relaxation / MGI
- neuronal intranuclear inclusions / MGI
- abnormal myocardial fiber physiology / MGI
- abnormal Paneth cell morphology / MGI
- decreased B cell number / MGI
- decreased cardiac muscle contractility / MGI
- glomerulosclerosis / MGI
- abnormal podocyte morphology / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- taste/olfaction phenotype / MGI
- hematopoietic system phenotype / MGI
- jerky movement / MGI
- thrombocytosis / MGI
- decreased ventricle muscle contractility / MGI
- decreased mean corpuscular hemoglobin concentration / MGI
- decreased dopamine level / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- absent podocyte slit diaphragm / MGI
- podocyte foot process effacement / MGI
- increased megakaryocyte cell number / MGI
- abnormal spatial reference memory / MGI
- abnormal spatial working memory / MGI
- abnormal splenic cell ratio / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal enterocyte proliferation / MGI
- abnormal enterocyte apoptosis / MGI
- abnormal neuron differentiation / MGI
- increased mammary gland tumor incidence / MGI
- myeloid hyperplasia / MGI
- expanded mesangial matrix / MGI
- mesangial cell hyperplasia / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- decreased brain choline acetyltransferase activity / MGI
- decreased brain tyrosine 3-monooxygenase activity / MGI
- decreased vascular endothelial cell proliferation / MGI
Literature references
- Mosaic complementation demonstrates a regulatory role for myosin VIIa in actin dynamics of stereocilia.;Prosser Haydn M, Rzadzinska Agnieszka K, Steel Karen P, Bradley Allan, ;2008;Molecular and cellular biology;28;1702-12; 18160714
- Multi-isotope imaging mass spectrometry reveals slow protein turnover in hair-cell stereocilia.;Zhang Duan-Sun, Piazza Valeria, Perrin Benjamin J, Rzadzinska Agnieszka K, Poczatek J Collin, Wang Mei, Prosser Haydn M, Ervasti James M, Corey David P, Lechene Claude P, ;2012;Nature;481;520-4; 22246323