C57BL/6-Tg(UBC-Brainbow1.0L)35Mal/Orl
| Status | Available to order |
| EMMA ID | EM:06050 |
| Citation information | RRID:IMSR_EM:06050 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(UBC-Brainbow1.0L)35Mal/Orl |
| Alternative name | Ubow |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(UBC-Brainbow1.0L)35Mal |
| Gene/Transgene symbol | Tg(UBC-Brainbow1.0L)35Mal |
Information from provider
| Provider | Marc BAJENOFF |
| Provider affiliation | null, Centre D'immunologie Marseille-Luminy |
| Genetic information | The Ubow transgene expression cassette consists in a 11.7 kb fragment containing the human ubiquitin C promoter region (ENSG00000150991), followed by a Splice Donor-Intron-Splice Acceptor cassette of 0.6 kb extracted from pDOI5 and fused to the Brainbow1.0L cassette (Cytobow) of 3.1 kb provided by J. Livet. Briefly, the Braibow1.0L cassette consists in a succession of coding sequences for fluorescent proteins (Tomato, CFP and EYFP) flanked by loxP/lox2272 recombination sites. |
| Phenotypic information | Before cre mediated recombination, all Ubow cells are red (tomato expression) and become yellow (YFP) or blue (CFP) upon cre induction. In mice homozygous for the Ubow transgene, cre expressing cells can be: tomato/tomato (red), tomato/YFP (orange), tomato/CFP (purple), YFP/YFP (yellow), CFP/CFP (blue) and CFP/YFP (green). Once acquired, the set of colors is definitive, allowing fate mapping and lineage tracing studies. |
| Breeding history | Ubow mice were generated on C57BL/6 background and were maintained on this background until then. They are currently bred as heterozygous x wild-type mice in our facility but homozygous x homozygous mice are breeding equally well. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J males |
Literature references
- Multicolor fate mapping of Langerhans cell homeostasis.;Ghigo Clément, Mondor Isabelle, Jorquera Audrey, Nowak Jonathan, Wienert Stephan, Zahner Sonja P, Clausen Björn E, Luche Hervé, Malissen Bernard, Klauschen Frederick, Bajénoff Marc, ;2013;The Journal of experimental medicine;210;1657-64; 23940255