Cnbc:NMRI-Tg(Wap-ALPP*)p1435Lmon

Status

Available to order

EMMA IDEM:06055
Citation informationRRID:IMSR_EM:06055 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameCnbc:NMRI-Tg(Wap-ALPP*)p1435Lmon
Alternative namestock HsdWin:NMRI-Tg(Wap-hSEAP)p1435Lmon
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Wap-ALPP*)p1435Lmon, Tyrc
Gene/Transgene symbolTg(Wap-ALPP*)p1435Lmon, Tyr

Information from provider

ProviderLluis Montoliu
Provider affiliationDepartamento de Biologia Molecular y Celular, CNB-CSIC, Centro Nacional de Biotecnologia
Genetic informationPlasmid pWap-SEAP, carrying 7.6 kb of mouse Wap sequences and the human SEAP reporter gene (Clontech) was injected into pronuclei of NMRI fertilized oocytes and resulted in transgenic mice founder line 1435, with >60 copies of transgene and no expression levels (chromosome position effects) in mammary gland, but high ectopic expression in kidney, muscle and lungs
Phenotypic informationNo specific traits distinguishable from NMRI mice other than expression data reported.
Breeding historyCreated and maintained in stock outbred albino HsdWin:NMRI
References
  • Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.;Millot Benjamin, Montoliu Lluís, Fontaine Marie-Louise, Mata Teresa, Devinoy Eve, ;2003;The Biochemical journal;372;41-52; 12580766
  • Design of vectors for transgene expression: The use of genomic comparative approaches.;Montoliu Lluís, Roy Rosa, Regales Lucía, García-Díaz Angel, ;2009;Comparative immunology, microbiology and infectious diseases;32;81-90; 18328563
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • absent coat pigmentation / MGI
  • diluted coat color / MGI
  • abnormal coat/hair pigmentation / MGI
  • absent eye pigmentation / MGI
  • abnormal coat appearance / MGI
  • decreased eye pigmentation / MGI
  • mottled coat / MGI
  • abnormal eye pigmentation / MGI
  • belly spot / MGI
  • hypopigmentation / MGI
  • variegated eye pigmentation pattern / MGI
  • decreased ear pigmentation / MGI
MGI phenotypes (gene matching)
  • abnormal cell morphology / MGI
  • diluted coat color / MGI
  • irregular coat pigmentation / MGI
  • belly spot / MGI
  • absent hair follicle melanin granules / MGI
  • shiny fur / MGI
  • mottled coat / MGI
  • abnormal retinal photoreceptor morphology / MGI
  • pigmentation phenotype / MGI
  • absent skin pigmentation / MGI
  • abnormal keratinocyte apoptosis / MGI
  • abnormal eye pigmentation / MGI
  • abnormal retina morphology / MGI
  • retinal degeneration / MGI
  • decreased retinal photoreceptor cell number / MGI
  • abnormal coat appearance / MGI
  • male infertility / MGI
  • abnormal coat/hair pigmentation / MGI
  • prenatal lethality / MGI
  • premature death / MGI
  • abnormal vision / MGI
  • abnormal skin pigmentation / MGI
  • no abnormal phenotype detected / MGI
  • no phenotypic analysis / MGI
  • abnormal cell nucleus morphology / MGI
  • failure of zygotic cell division / MGI
  • single kidney / MGI
  • absent seminal vesicle / MGI
  • abnormal chromosome morphology / MGI
  • chromosome breakage / MGI
  • induced chromosome breakage / MGI
  • increased cellular sensitivity to ionizing radiation / MGI
  • abnormal miscarriage rate / MGI
  • abnormal hair follicle melanogenesis / MGI
  • abnormal melanosome morphology / MGI
  • abnormal iris pigmentation / MGI
  • absent coat pigmentation / MGI
  • decreased eye pigmentation / MGI
  • abnormal aqueous drainage system morphology / MGI
  • abnormal retinal ganglion layer morphology / MGI
  • abnormal eye physiology / MGI
  • abnormal intraocular pressure / MGI
  • variegated coat color / MGI
  • homeostasis/metabolism phenotype / MGI
  • reproductive system phenotype / MGI
  • vision/eye phenotype / MGI
  • hypopigmentation / MGI
  • ocular albinism / MGI
  • absent eye pigmentation / MGI
  • decreased survivor rate / MGI
  • transverse fur striping / MGI
  • mortality/aging / MGI
  • abnormal survival / MGI
  • integument phenotype / MGI
  • neonatal lethality, complete penetrance / MGI
  • perinatal lethality, complete penetrance / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality, complete penetrance / MGI
  • embryonic lethality at implantation, complete penetrance / MGI
  • embryonic lethality before implantation, complete penetrance / MGI
  • decreased ear pigmentation / MGI
  • variegated eye pigmentation pattern / MGI
  • decreased a wave amplitude / MGI
  • decreased b wave amplitude / MGI

Literature references

  • Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.;Millot Benjamin, Montoliu Lluís, Fontaine Marie-Louise, Mata Teresa, Devinoy Eve, ;2003;The Biochemical journal;372;41-52; 12580766
  • Design of vectors for transgene expression: The use of genomic comparative approaches.;Montoliu Lluís, Roy Rosa, Regales Lucía, García-Díaz Angel, ;2009;Comparative immunology, microbiology and infectious diseases;32;81-90; 18328563

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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