Cnbc:NMRI-Tg(Wap-ALPP*)b6733Lmon

Status	Available to order
EMMA ID	EM:06059
Citation information	RRID:IMSR_EM:06059 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	Cnbc:NMRI-Tg(Wap-ALPP*)b6733Lmon
Alternative name	stock HsdWin:NMRI-Tg(Wap-hSEAP)b6733Lmon
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Wap-ALPP*)b6733Lmon, Tyr^c
Gene/Transgene symbol	Tg(Wap-ALPP*)b6733Lmon, Tyr

Information from provider

Provider	Lluis Montoliu
Provider affiliation	Departamento de Biologia Molecular y Celular, CNB-CSIC, Centro Nacional de Biotecnologia
Genetic information	BAC bWap-SEAP, carrying 24 kb of mouse Wap sequences and the human SEAP reporter gene (Clontech) was injected into pronuclei of NMRI fertilized oocytes and resulted in transgenic mice founder line 6733, with 2 copies of transgene and expression levels in mammary gland of 5.1 µg/ml milk, and some detectable ectopic expression in liver and brain.
Phenotypic information	No specific traits distinguishable from NMRI mice other than expression data reported
Breeding history	Created and maintained in stock outbred albino HsdWin:NMRI
References	Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.;Millot Benjamin, Montoliu Lluís, Fontaine Marie-Louise, Mata Teresa, Devinoy Eve, ;2003;The Biochemical journal;372;41-52; 12580766 Design of vectors for transgene expression: The use of genomic comparative approaches.;Montoliu Lluís, Roy Rosa, Regales Lucía, García-Díaz Angel, ;2009;Comparative immunology, microbiology and infectious diseases;32;81-90; 18328563
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Temperature-sensitive oculocutaneous albinism type 1 / Orphanet_352737
Oculocutaneous albinism type 1A / Orphanet_79431
Oculocutaneous albinism type 1B / Orphanet_79434
Minimal pigment oculocutaneous albinism type 1 / Orphanet_352734
Ocular albinism with congenital sensorineural deafness / Orphanet_352740

MGI phenotypes (allele matching)

absent coat pigmentation / MGI
diluted coat color / MGI
abnormal coat/hair pigmentation / MGI
absent eye pigmentation / MGI
abnormal coat appearance / MGI
decreased eye pigmentation / MGI
mottled coat / MGI
abnormal eye pigmentation / MGI
belly spot / MGI
hypopigmentation / MGI
variegated eye pigmentation pattern / MGI
decreased ear pigmentation / MGI

MGI phenotypes (gene matching)

abnormal cell morphology / MGI
diluted coat color / MGI
irregular coat pigmentation / MGI
belly spot / MGI
absent hair follicle melanin granules / MGI
shiny fur / MGI
mottled coat / MGI
abnormal retinal photoreceptor morphology / MGI
pigmentation phenotype / MGI
absent skin pigmentation / MGI
abnormal keratinocyte apoptosis / MGI
abnormal eye pigmentation / MGI
abnormal retina morphology / MGI
retinal degeneration / MGI
decreased retinal photoreceptor cell number / MGI
abnormal coat appearance / MGI
male infertility / MGI
abnormal coat/hair pigmentation / MGI
prenatal lethality / MGI
premature death / MGI
abnormal vision / MGI
abnormal skin pigmentation / MGI
no abnormal phenotype detected / MGI
no phenotypic analysis / MGI
abnormal cell nucleus morphology / MGI
failure of zygotic cell division / MGI
single kidney / MGI
absent seminal vesicle / MGI
abnormal chromosome morphology / MGI
chromosome breakage / MGI
induced chromosome breakage / MGI
increased cellular sensitivity to ionizing radiation / MGI
abnormal miscarriage rate / MGI
abnormal hair follicle melanogenesis / MGI
abnormal melanosome morphology / MGI
abnormal iris pigmentation / MGI
absent coat pigmentation / MGI
decreased eye pigmentation / MGI
abnormal aqueous drainage system morphology / MGI
abnormal retinal ganglion layer morphology / MGI
abnormal eye physiology / MGI
abnormal intraocular pressure / MGI
variegated coat color / MGI
homeostasis/metabolism phenotype / MGI
reproductive system phenotype / MGI
vision/eye phenotype / MGI
hypopigmentation / MGI
ocular albinism / MGI
absent eye pigmentation / MGI
decreased survivor rate / MGI
transverse fur striping / MGI
mortality/aging / MGI
abnormal survival / MGI
integument phenotype / MGI
neonatal lethality, complete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality at implantation, complete penetrance / MGI
embryonic lethality before implantation, complete penetrance / MGI
decreased ear pigmentation / MGI
variegated eye pigmentation pattern / MGI
decreased a wave amplitude / MGI
decreased b wave amplitude / MGI

Literature references

Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.;Millot Benjamin, Montoliu Lluís, Fontaine Marie-Louise, Mata Teresa, Devinoy Eve, ;2003;The Biochemical journal;372;41-52; 12580766
Design of vectors for transgene expression: The use of genomic comparative approaches.;Montoliu Lluís, Roy Rosa, Regales Lucía, García-Díaz Angel, ;2009;Comparative immunology, microbiology and infectious diseases;32;81-90; 18328563

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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