B6.Cg-Tg(Alb-Egfr*)5026Ciem/Cnbc
| Status | Available to order |
| EMMA ID | EM:06072 |
| Citation information | RRID:IMSR_EM:06072 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Alb-Egfr*)5026Ciem/Cnbc |
| Alternative name | B6.B6CBA-Tg(Alb-DeltaEGFR)5026Ciem |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Alb-Egfr*)5026Ciem |
| Gene/Transgene symbol | Tg(Alb-Egfr*)5026Ciem |
Information from provider
| Provider | Jose Carlos Segovia |
| Provider affiliation | INVESTIGACION BASICA, CIEMAT |
| Genetic information | DeltaEGFR cDNA was cloned under the control of the albumin mouse locus harbored in BAC clone RP23-27P6 (chrom. 5) by recombineering techniques (up to 160 kbp of this construct were injected). Three transgenic mouse lines were produced; this description corresponds to line 5026, with 10-20 copies. These mice are, currently, in the fifth generation of backcrossing to C57BL/6J. |
| Phenotypic information | Very low or absent activation of Egfr upon Egf stimulus in isolated adult hepatocytes analyzed by Western blot. Attenuation of Egfr phosphorylation in liver after partial hepatectomy detected by immunohistochemistry. |
| Breeding history | Founders were produced in B6CBAF2 background. At the moment, animals are in the fifth generation of backcross to C57BL/6J background. |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous (C57BL/6 x CBA)F2 males |