B6.Cg-Fcgr2b^tm1.2Jsv/Cnbc

Status	Available to order
EMMA ID	EM:06078
Citation information	RRID:IMSR_EM:06078 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.Cg-Fcgr2b^tm1.2Jsv/Cnbc
Alternative name	Fcgr2bB6null
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Fcgr2b^tm1.2Jsv
Gene/Transgene symbol	Fcgr2b

Information from provider

Provider	Sjef Verbeek
Provider affiliation	Human Genetics, Leiden University Medical Center (LUMC)
Genetic information	By homologous recombination loxP sites were introduced in intron 3 (between EC1, extracellular domain1-encoding exon, and EC2, extracellular domain 2-encoding exon) and intron 5 (between TM, transmembrane and C1, intracellular domain 1-encoding exon). By crossing the floxed FcgRIIb-containing strain with the EIIA-cre deleter strain (backcrossed to C57BL/6 background) the Fcgr2bB6-null was obtained.
Phenotypic information	Exhibit a hyperactive phenotype characterized by elevated antibody titers, increased hypersensitivity, and susceptibility to collagen-induced arthritis but importantly NOT spontaneous autoimmunity (lupus), as it was described for the Fcgr2b knock-out generated by gene targeting in 129-derived ES cells and subsequently backcrossed for more than 6 generations to C57BL/6.
Breeding history	Chimera crossed to C57BL/6; F1 crossed to EIIa-cre deleter (backcrossed 12 generations to C57BL/6); F2 backcrossed to C57BL/6; F3 backcrossed to C57BL/6; F4 intercrossed and F5 selected for homozygous knock-out mice.
References	The inhibiting Fc receptor for IgG, FcγRIIB, is a modifier of autoimmune susceptibility.;Boross Peter, Arandhara Victoria L, Martin-Ramirez Javier, Santiago-Raber Marie-Laure, Carlucci Francesco, Flierman Roelof, van der Kaa Jos, Breukel Cor, Claassens Jill W C, Camps Marcel, Lubberts Erik, Salvatori Daniela, Rastaldi Maria Pia, Ossendorp Ferry, Daha Mohamed R, Cook H Terence, Izui Shozo, Botto Marina, Verbeek J Sjef, ;2011;Journal of immunology (Baltimore, Md. : 1950);187;1304-13; 21724994
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	yes

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous C57BL/6 males, homozygous C57BL/6 females
Stage of embryos	4/8-cell

Disease and phenotype information

MGI phenotypes (gene matching)

enlarged spleen / MGI
enlarged lymph nodes / MGI
decreased body weight / MGI
dehydration / MGI
hunched posture / MGI
anemia / MGI
abnormal seizure response to electrical stimulation / MGI
edema / MGI
increased inflammatory response / MGI
kidney inflammation / MGI
liver inflammation / MGI
lung inflammation / MGI
vasculitis / MGI
pancreas inflammation / MGI
salivary gland inflammation / MGI
premature death / MGI
abnormal hypersensitivity reaction / MGI
no abnormal phenotype detected / MGI
maximal tonic hindlimb extension seizures / MGI
abnormal mast cell physiology / MGI
increased immunoglobulin level / MGI
increased IgG level / MGI
increased IgM level / MGI
increased IgA level / MGI
glomerulonephritis / MGI
increased urine protein level / MGI
increased susceptibility to induced arthritis / MGI
tonic-clonic seizures / MGI
increased anti-double stranded DNA antibody level / MGI
increased anti-chromatin antibody level / MGI
decreased susceptibility to parasitic infection / MGI
skeleton phenotype / MGI
increased susceptibility to type I hypersensitivity reaction / MGI
increased germinal center B cell number / MGI
increased IgG1 level / MGI
increased IgG2a level / MGI
increased IgG2b level / MGI
increased IgG3 level / MGI
decreased B cell apoptosis / MGI
mortality/aging / MGI
renal glomerular protein deposits / MGI

Literature references

The inhibiting Fc receptor for IgG, FcγRIIB, is a modifier of autoimmune susceptibility.;Boross Peter, Arandhara Victoria L, Martin-Ramirez Javier, Santiago-Raber Marie-Laure, Carlucci Francesco, Flierman Roelof, van der Kaa Jos, Breukel Cor, Claassens Jill W C, Camps Marcel, Lubberts Erik, Salvatori Daniela, Rastaldi Maria Pia, Ossendorp Ferry, Daha Mohamed R, Cook H Terence, Izui Shozo, Botto Marina, Verbeek J Sjef, ;2011;Journal of immunology (Baltimore, Md. : 1950);187;1304-13; 21724994

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.Cg-Fcgr2btm1.2Jsv/Cnbc