C57BL/6J-Dckmemi/H

Status

Available to order

EMMA IDEM:06116
Citation informationRRID:IMSR_EM:06116 

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International strain nameC57BL/6J-Dckmemi/H
Alternative namedCKmemi
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolDckmemi
Gene/Transgene symbolDck

Information from provider

ProviderSophie Rutschmann
Provider affiliationMedicine, Imperial College London
Genetic informationAn ENU mutagenesis in a pure C57BL/6 background has induced a G to T transversion in the gene coding for deoxycytidine kinase (Dck) that results in a STOP codon for glutamic acid at position 247 (E247X). The mutant protein is functionally null.
Phenotypic informationA deficiency in deoxycytidine kinase affects peripheral T cell homeostatic proliferation and survival. Both B and T lymphocyte development is impaired, leading to a state of chronic lymphopenia and to a significant increase in the number of myeloid cells and erythrocytes. In the periphery, mutant lymphocytes adopt a memory phenotype, with high levels of proliferation and apoptosis, due to both cell-extrinsic and -intrinsic effects of the mutation.
Breeding historyInbred, G12
References
  • A deficiency in nucleoside salvage impairs murine lymphocyte development, homeostasis, and survival.;Choi Onjee, Heathcote Dean A, Ho Ka-Kei, Müller Phillip J, Ghani Hazim, Lam Eric W-F, Ashton-Rickardt Philip G, Rutschmann Sophie, ;2012;Journal of immunology (Baltimore, Md. : 1950);188;3920-7; 22407915
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)
  • enlarged spleen / MGI
  • small thymus / MGI
  • thymus hypoplasia / MGI
  • abnormal T cell differentiation / MGI
  • increased erythroid progenitor cell number / MGI
  • decreased B cell number / MGI
  • abnormal response to infection / MGI
  • increased T cell proliferation / MGI
  • increased T cell apoptosis / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • abnormal myeloid leukocyte morphology / MGI
  • increased effector memory CD4-positive, alpha-beta T cell number / MGI
  • increased effector memory CD8-positive, alpha-beta T cell number / MGI
MGI phenotypes (gene matching)
  • extramedullary hematopoiesis / MGI
  • abnormal spleen morphology / MGI
  • enlarged spleen / MGI
  • abnormal thymus morphology / MGI
  • small thymus / MGI
  • impaired hematopoiesis / MGI
  • decreased IgG level / MGI
  • thymus hypoplasia / MGI
  • abnormal T cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • abnormal double-negative T cell morphology / MGI
  • increased IgE level / MGI
  • reticulocytosis / MGI
  • increased erythroid progenitor cell number / MGI
  • increased spleen weight / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • abnormal response to infection / MGI
  • absent spleen white pulp / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • increased T cell proliferation / MGI
  • increased macrophage cell number / MGI
  • increased T cell apoptosis / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • increased pro-B cell number / MGI
  • decreased pre-B cell number / MGI
  • abnormal myeloid leukocyte morphology / MGI
  • abnormal peripheral lymph node morphology / MGI
  • increased effector memory CD4-positive, alpha-beta T cell number / MGI
  • increased effector memory CD8-positive, alpha-beta T cell number / MGI
  • decreased response to antigen / MGI

Literature references

  • A deficiency in nucleoside salvage impairs murine lymphocyte development, homeostasis, and survival.;Choi Onjee, Heathcote Dean A, Ho Ka-Kei, Müller Phillip J, Ghani Hazim, Lam Eric W-F, Ashton-Rickardt Philip G, Rutschmann Sophie, ;2012;Journal of immunology (Baltimore, Md. : 1950);188;3920-7; 22407915

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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