C57BL/6-Tg(IVL-tdTomato)1Rase/Ph

Status	Available to order
EMMA ID	EM:06136
Citation information	RRID:IMSR_EM:06136 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C57BL/6-Tg(IVL-tdTomato)1Rase/Ph
Alternative name	C57Bl/6-Tg(Inv-tdTomato)Rase
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(IVL-tdTomato)1Rase
Gene/Transgene symbol	Tg(IVL-tdTomato)1Rase

Information from provider

Provider	Radislav Sedlacek
Provider affiliation	Department of Transgenic Models of Diseases, Institute of Molecular Genetics
Genetic information	Construct consisting of human involucrin truncated promoter, first involucrin intron, tdTomato cDNA, and beta-globin polyA signal (total 3707 bp) was injected into pronuclei of fertilized oocytes to generate transgenic mice.
Phenotypic information	Expression of tdTomato fluorescent protein (578 nm) in outermost epidermal layers (stratum spinosum, stratum granulare and partly corneum) and inner root sheet of hair follicles. Weak expression in tongue and bladder.
Breeding history	Positive transgenic mice are paired with C57BL/6N partners. Currently G2 mice are in breeding.
References	Transgenic mouse model expressing tdTomato under involucrin promoter as a tool for analysis of epidermal differentiation and wound healing.;Kasparek Petr, Krenek Pavel, Buryova Halka, Suchanova Sarka, Beck Inken Maria, Sedlacek Radislav, ;2012;Transgenic research;21;683-9; 22020981
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Institute of Molecular Genetics, Prague, Czech Republic
Animals used for archiving	heterozygous C57BL/6NCrl males, wild-type C57BL/6NCrl females
Breeding at archiving centre	Transgenic tdTomato males were bred with C57BL/6N females to obtain proven breeder males. These males were taken to carry out IVF for freezing 2-cells stage embryos and to freeze sperm.
Stage of embryos	2-cell

Literature references

Transgenic mouse model expressing tdTomato under involucrin promoter as a tool for analysis of epidermal differentiation and wound healing.;Kasparek Petr, Krenek Pavel, Buryova Halka, Suchanova Sarka, Beck Inken Maria, Sedlacek Radislav, ;2012;Transgenic research;21;683-9; 22020981

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.