B6;D2-Tg(Tff3/MUC5B)208Jlcd/Cnbc

Status	Available to order
EMMA ID	EM:06336
Citation information	RRID:IMSR_EM:06336 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;D2-Tg(Tff3/MUC5B)208Jlcd/Cnbc
Alternative name	K208
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Tff3/MUC5B)208Jlcd
Gene/Transgene symbol	Tg(Tff3/MUC5B)208Jlcd

Information from provider

Provider	Jean-Luc Desseyn
Provider affiliation	U995-4, Inserm
Genetic information	Mouse Tff3 promoter plus Tff3 exons 1 and 2 (with full intron 1 and part of intron 2), followed by an artificial exon 3 encoding 12 copies of the CYS domain#4 of the human MUC5B gene followed by a 3'-end sequence. Construct (insert) spanning 15.2 kb.
Phenotypic information	Expression of the transgene similar to Tff3. Modification of the mucus layer in the intestine. Better protection (mucus reinforcement) against pathogens. Higher load of Lactobacilli.
Breeding history	3 backcrosses to C57BL/6J and then intercrosses to obtain homozygous line. The homozygous status was determined when obtaining 100% transgenic pups from the breeding of one putative homozygous to C57BL/6.
References	Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier.;Gouyer Valérie, Dubuquoy Laurent, Robbe-Masselot Catherine, Neut Christel, Singer Elisabeth, Plet Ségolène, Geboes Karel, Desreumaux Pierre, Gottrand Frédéric, Desseyn Jean-Luc, ;2015;Scientific reports;5;9577; 25974250
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	homozygous (C57BL/6 x DBA/2)F2 males, wild-type (C57BL/6 x DBA/2)F1 females
Stage of embryos	2-cell

Literature references

Delivery of a mucin domain enriched in cysteine residues strengthens the intestinal mucous barrier.;Gouyer Valérie, Dubuquoy Laurent, Robbe-Masselot Catherine, Neut Christel, Singer Elisabeth, Plet Ségolène, Geboes Karel, Desreumaux Pierre, Gottrand Frédéric, Desseyn Jean-Luc, ;2015;Scientific reports;5;9577; 25974250

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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