STOCK Tg(Alb-luc/EGFP)5189Ciem/Cnbc

Status	Available to order
EMMA ID	EM:06344
Citation information	RRID:IMSR_EM:06344 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(Alb-luc/EGFP)5189Ciem/Cnbc
Alternative name	C,B6CBA-Tg(Alb-Luc-eGFP)5189Ciem [Alb1-LUC-eGFP.5189]
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Alb-luc/EGFP)5189Ciem
Gene/Transgene symbol	Tg(Alb-luc/EGFP)5189Ciem

Information from provider

Provider	Jose Carlos Segovia
Provider affiliation	Differentiation and Cytometry Unit. Hematopoietic and Gene Therapy Division, Centro de Investigaciones Energéticas, Medio Ambientales y Tecnológicas (CIEMAT)
Genetic information	Luciferase-eGFP fusion reporter gene inserted at ATG start codon of Alb1 mouse locus harbored in BAC clone RP23-27P6 (chromosome 5) by recombineering techniques (160 kpb of this construction injected). The utility of the Alb1-LUC:eGFP transgenic mouse includes: (1) Monitoring of hepatic damage/recovery by non-invasive techniques - this would be useful in studies of drug hepatotoxicity/screening; (2) tracking of bone marrow donor cells that could contribute to hepatic regeneration in a context of liver injury. Copy number of Alb1-LUC-eGFP.5189 transgene (qPCR): 8-9.
Phenotypic information	Alb1-LUC-eGFP.5189: - specific high luciferase activity in liver (less than 1% luciferase activity in other tissues) - specific EGFP staining in cryosections of liver - high in vivo liver specific luminescence detected after treatment with luciferine - liver-specific epi-fluorescence detected
Breeding history	Founders are in background B6CBAF2. Current animals (F2) are in the 2nd generation of backcrossing to BALB/c background.
References	None available
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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