B6;CBA-Tg(Chek1)1Ofc/Cnbc
| Status | Available to order |
| EMMA ID | EM:06789 |
| Citation information | RRID:IMSR_EM:06789 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;CBA-Tg(Chek1)1Ofc/Cnbc |
| Alternative name | Chek1 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Chek1)1Ofc |
| Gene/Transgene symbol | Tg(Chek1)1Ofc |
Information from provider
| Provider | Oskar Fernandez-Capetillo |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas (CNIO) |
| Genetic information | The transgene contains a 33.5 kb murine BAC segment with the centrally located gene of interest. The BAC used is: Rp23-318C6. The 33.5 kb region from the mouse genome includes Chk1 and sequences 5' and 3' of the gene until the next conserved gene was found in either direction. For the generation of Chk1 Tg mice, this 33.5-kb region was first cloned into a minimal vector by recombineering. The linearized vector was used for the microinjection of fertilized oocytes The BAC transgene (Chek1Tg) is described in the original reference (PubMed ID: 22370720) |
| Phenotypic information | Transgenic mice are viable with no obvious phenotype that will distinguish them from their littermates. In vitro, Chk1 transgenic cells are protected from replicative stress -inducing agents. Moreover, an extra Chk1 allele prolongs the survival of ATR-Seckel mice, which suffer from high levels of replicative stress, but not that of ATM-deficient mice, which accumulate DNA breaks. Increased Chk1 levels favour transformation, which is associated with a reduction in the levels of replicative stress induced by oncogenes. |
| Breeding history | Founder was crossed with C57BL/6J animals. The line is currently maintained by crossing hemizygous mice with C57BL/6J mice. Homozygous mice are viable but growth retardation and premature death occurs in homozygosis. Usually they do not reach breeding age. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J males, wild-type C57BL/6J females |
| Stage of embryos | 2-cell |
Literature references
- An extra allele of Chk1 limits oncogene-induced replicative stress and promotes transformation.;López-Contreras Andres J, Gutierrez-Martinez Paula, Specks Julia, Rodrigo-Perez Sara, Fernandez-Capetillo Oscar, ;2012;The Journal of experimental medicine;209;455-61; 22370720