STOCK Tg(CAG-Katushka)3Sgo/Cnbc

Status	Available to order
EMMA ID	EM:06790
Citation information	RRID:IMSR_EM:06790 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	STOCK Tg(CAG-Katushka)3Sgo/Cnbc
Alternative name	Tg.CAG-LSL-KFP, Tg(CAG-Katushka)3Sgo
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CAG-Katushka)3Sgo
Gene/Transgene symbol	Tg(CAG-Katushka)3Sgo

Information from provider

Provider	Sagrario Ortega
Provider affiliation	Biotechnology, Centro Nacional de Investigaciones Oncologicas
Genetic information	A 4.7 Kb DNA fragment of a plasmid containing the CAG promoter followed by a transcription/translation STOP sequence flanked by directly repeated loxP sites, the Katushka reporter cDNA from pTurboFP635-C (Evrogen) and the rabbit O-globin polyadenylation signal, was injected into B6xCBA oocytes. This is a cre recombinase reporter line. After cre-mediated excision of the floxed STOP sequences, the Katushka far-red fluorescent protein (KFP) reporter is expressed under the control of the CAG ubiquitous promoter. This strain derives from founder #3.
Phenotypic information	Mice do not have any significant phenotype.
Breeding history	The cre-reporter founder animals were crossed with B6(Cg)-Tyr^c-2J/J animals for testing germ-line transmission. The line is maintained by crossing heterozygous animals with B6(Cg)-Tyr^c-2J/J mice.
References	A Cre-reporter transgenic mouse expressing the far-red fluorescent protein Katushka.;Diéguez-Hurtado Rodrigo, Martín Javier, Martínez-Corral Inés, Martínez María Dolores, Megías Diego, Olmeda David, Ortega Sagrario, ;2011;Genesis (New York, N.Y. : 2000);49;36-45; 21254335
Homozygous fertile	not known
Homozygous viable	not known
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous C57BL/6J males

Literature references

A Cre-reporter transgenic mouse expressing the far-red fluorescent protein Katushka.;Diéguez-Hurtado Rodrigo, Martín Javier, Martínez-Corral Inés, Martínez María Dolores, Megías Diego, Olmeda David, Ortega Sagrario, ;2011;Genesis (New York, N.Y. : 2000);49;36-45; 21254335

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Right strain for your research?

The information provided on this page is, to the best of EMMA’s knowledge, based on data supplied by the original provider. End users are responsible for reviewing these details and for validating the strain and its suitability for their experimental use.
Not found what you were looking for? Search here for other strains available from EMMA.