IMPC phenotypes (gene matching)
B6.129(CD1)-Cdk6tm1Bbd/Cnbc
| Status | Available to order |
| EMMA ID | EM:06839 |
| Citation information | RRID:IMSR_EM:06839 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129(CD1)-Cdk6tm1Bbd/Cnbc |
| Alternative name | Cdk6 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cdk6tm1Bbd |
| Gene/Transgene symbol | Cdk6 |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | A PGK-neo cassette replaced the first coding exon, which contains the ATG as well as the PLSTIRE domain essential for cyclin binding. PCR confirmed recombination in mutant mice. Immunohistochemical analysis of lymphocytes indicated lack of protein expression in mutants. |
| Phenotypic information | Heterozygous animals do not exhibit any phenotype. Homozygous mice show abnormal hematopoietic system morphology/development, decreased T cell proliferation, abnormal spleen and thymus morphology and reduced female fertility (approximately 30% of the females are sterile). |
| Breeding history | Chimeras were initially crossed with CD1 females for germ line transmission analysis. The line has been backcrossed into C57BL/6J for 10 generations. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J males |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Autosomal recessive primary microcephaly / Orphanet_2512
MGI phenotypes (allele matching)
- decreased leukocyte cell number / MGI
- abnormal spleen morphology / MGI
- small spleen / MGI
- spleen hypoplasia / MGI
- abnormal thymus morphology / MGI
- decreased body weight / MGI
- thymus hypoplasia / MGI
- reduced female fertility / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal spleen red pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal erythrocyte morphology / MGI
- increased mean corpuscular volume / MGI
- decreased mature ovarian follicle number / MGI
- decreased erythrocyte cell number / MGI
- decreased T cell proliferation / MGI
- increased mean corpuscular hemoglobin concentration / MGI
- increased single-positive T cell number / MGI
- decreased megakaryocyte cell number / MGI
- thymus cortex atrophy / MGI
MGI phenotypes (gene matching)
- decreased leukocyte cell number / MGI
- abnormal spleen morphology / MGI
- small spleen / MGI
- spleen hypoplasia / MGI
- abnormal thymus morphology / MGI
- small thymus / MGI
- enlarged thymus / MGI
- increased thymocyte number / MGI
- decreased thymocyte number / MGI
- decreased body weight / MGI
- thymus hypoplasia / MGI
- reduced female fertility / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal T cell differentiation / MGI
- abnormal spleen red pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal lymphopoiesis / MGI
- abnormal erythrocyte morphology / MGI
- increased mean corpuscular volume / MGI
- decreased mature ovarian follicle number / MGI
- decreased erythrocyte cell number / MGI
- thymus atrophy / MGI
- abnormal thymocyte activation / MGI
- increased hematopoietic stem cell number / MGI
- decreased hematopoietic stem cell number / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased double-positive T cell number / MGI
- decreased T cell proliferation / MGI
- immune system phenotype / MGI
- increased mean corpuscular hemoglobin concentration / MGI
- increased T cell apoptosis / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- increased single-positive T cell number / MGI
- decreased megakaryocyte cell number / MGI
- decreased thymocyte apoptosis / MGI
- increased DN1 thymic pro-T cell number / MGI
- decreased DN1 thymic pro-T cell number / MGI
- decreased DN2 thymocyte number / MGI
- increased DN3 thymocyte number / MGI
- decreased DN4 thymocyte number / MGI
- abnormal hematopoietic stem cell physiology / MGI
- thymus cortex atrophy / MGI
Literature references
- Cdk1 is sufficient to drive the mammalian cell cycle.;Santamaría David, Barrière Cédric, Cerqueira Antonio, Hunt Sarah, Tardy Claudine, Newton Kathryn, Cáceres Javier F, Dubus Pierre, Malumbres Marcos, Barbacid Mariano, ;2007;Nature;448;811-5; 17700700
- A synthetic lethal interaction between K-Ras oncogenes and Cdk4 unveils a therapeutic strategy for non-small cell lung carcinoma.;Puyol Marta, Martín Alberto, Dubus Pierre, Mulero Francisca, Pizcueta Pilar, Khan Gulfaraz, Guerra Carmen, Santamaría David, Barbacid Mariano, ;2010;Cancer cell;18;63-73; 20609353
- Mammalian cells cycle without the D-type cyclin-dependent kinases Cdk4 and Cdk6.;Malumbres Marcos, Sotillo Rocío, Santamaría David, Galán Javier, Cerezo Ana, Ortega Sagrario, Dubus Pierre, Barbacid Mariano, ;2004;Cell;118;493-504; 15315761
- Cell cycle and cancer: genetic analysis of the role of cyclin-dependent kinases.;Barbacid M, Ortega S, Sotillo R, Odajima J, Martín A, Santamaría D, Dubus P, Malumbres M, ;2005;Cold Spring Harbor symposia on quantitative biology;70;233-40; 16869759