MGI phenotypes (allele matching)
B6;129S2-Cdk7Gt(D032B11)Wrst/Cnbc
| Status | Available to order |
| EMMA ID | EM:06840 |
| Citation information | RRID:IMSR_EM:06840 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129S2-Cdk7Gt(D032B11)Wrst/Cnbc |
| Alternative name | Cdk7 |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Cdk7Gt(D032B11)Wrst |
| Gene/Transgene symbol | Cdk7 |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | The strain was obtained by microinjection of the genetrap (GGTC) D032B11 clone in C57BL/6J blastocyts. In this clone the genetrap vector inserted in intron 2, 1.52 kbp upstream of exon 3. The gene trap vector has pairs of FRT, F3, loxP and lox5171 sites allowing for inversion of the trapping cassette. Before Cre or Flp mediated recombination the allele is functionally equivalent to a knockout allele. |
| Phenotypic information | No phenotype in heterozygosis. Crosses between heterozygous animals failed to produce live Cdk7loxfrt/loxfrt offspring as well as E13.5 and E9.5 embryos. Homozygous embryos die at peri-implantation stage. Analysis of E5.5 embryos derived from these crosses revealed deciduas with a high percentage of apoptotic cells within the embryonic body that were negative for Cdk7 immunostaining. |
| Breeding history | Chimeras were crossed with C57BL/6J females for germ line transmission analysis. The line has been maintained by crossing heterozygous animals with C57BL/6J mice or by crossing heterozygous animals among themselves. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous males |
Disease and phenotype information
Literature references
- A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.;Hansen Jens, Floss Thomas, Van Sloun Petra, Füchtbauer Ernst-Martin, Vauti Franz, Arnold Hans-Hennig, Schnütgen Frank, Wurst Wolfgang, von Melchner Harald, Ruiz Patricia, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;9918-22; 12904583
- Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion.;Ganuza Miguel, Sáiz-Ladera Cristina, Cañamero Marta, Gómez Gonzalo, Schneider Ralph, Blasco María A, Pisano David, Paramio Jesús M, Santamaría David, Barbacid Mariano, ;2012;The EMBO journal;31;2498-510; 22505032