MGI phenotypes (gene matching)
STOCK Cdk7Gt(D032B11)1.1Wrst/Cnbc
| Status | Available to order |
| EMMA ID | EM:06841 |
| Citation information | RRID:IMSR_EM:06841 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Cdk7Gt(D032B11)1.1Wrst/Cnbc |
| Alternative name | Cdk7 |
| Strain type | Gene-trap |
| Allele/Transgene symbol | Cdk7Gt(D032B11)1.1Wrst |
| Gene/Transgene symbol | Cdk7 |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | The strain was obtained by microinjection of the genetrap (GGTC) D032B11 clone in C57BL/6J blastocyts. In this clone the genetrap vector inserted in intron 2, 1.52 kbp upstream of exon 3. The genetrap vector has pairs of FRT, F3, loxP and lox5171 sites allowing for inversion of the trapping cassette. In this strain, Flp mediated recombination inverted the genetrap vector in intron 2 allowing expression of the gene from the Cdk7lox allele. Upon Cre-mediated recombination, the genetrap vector can be inverted again interfering with gene expression. The Cdk7lox behaves like a Cre/lox conditional KO allele in this strain. |
| Phenotypic information | No phenotype in heterozygosis or homozygosis. The allele behaves as a true conditional knockout allele for Cdk7. |
| Breeding history | Chimeras were crossed with C57BL/6J females for germ line transmission analysis. Heterozygous animals carrying the original genetrap allele were crossed with a transgenic line expressing the Flp recombinase in the germ line (Rodríguez et al., 2000; PMID:10835623). The line has been maintained by crossing homozygous animals or heterozygous animals among themselves. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous males |
Disease and phenotype information
Literature references
- A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.;Hansen Jens, Floss Thomas, Van Sloun Petra, Füchtbauer Ernst-Martin, Vauti Franz, Arnold Hans-Hennig, Schnütgen Frank, Wurst Wolfgang, von Melchner Harald, Ruiz Patricia, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;9918-22; 12904583
- Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion.;Ganuza Miguel, Sáiz-Ladera Cristina, Cañamero Marta, Gómez Gonzalo, Schneider Ralph, Blasco María A, Pisano David, Paramio Jesús M, Santamaría David, Barbacid Mariano, ;2012;The EMBO journal;31;2498-510; 22505032
- Inhibition of cyclin-dependent kinase 7 mitigates doxorubicin cardiotoxicity and enhances anticancer efficacy.;Chen Jingrui, Wei Jing, Xia Peng, Liu Yuening, Belew Mahder Dawit, Toohill Ryan, Wu Boyang Jason, Cheng Zhaokang, ;2024;Cardiovascular research;120;1024-1036; 38646672