C57BL/6-Tg(CMV*-RFP1)6/Flmg
| Status | Available to order |
| EMMA ID | EM:07172 |
| Citation information | RRID:IMSR_EM:07172 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | C57BL/6-Tg(CMV*-RFP1)6/Flmg |
| Alternative name | B6-Tg (TRE-Rfp1) PS1/BRFAA (short name TRE-Rfp1) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CMV*-RFP1)6 |
| Gene/Transgene symbol | Tg(CMV*-RFP1)6 |
Information from provider
| Provider | Paschalis Sideras |
| Provider affiliation | Center of Immunology & Transplantation, Biomedical Research Foundation of the Academy of Athens (BRFAA) |
| Genetic information | In this reporter line, the RFP1 red fluorescent protein reporter is expressed under the control of a synthetic Smad2/3-induced promoter. The core element of this promoter consists of 12 repeats of the well characterized CAGA element, the Smad2/3 binding element (EMBO J. 1998 Jun 1;17:3091-100). The TGF-beta responsiveness of the DNA construct was evaluated in-culture in the presence of inducers or inhibitors of the TGF-beta signaling. |
| Phenotypic information | This line does not have any phenotypic alterations, compared to wild-type mice. It breeds normally and the weight curve during development is normal. It is a reporter line, expressing the red fluorescent protein RFP1 under the control of a synthetic Smad2/3-induced promoter. Lungs were removed and a single cell suspension was achieved after enzymatic digestion of the tissue. RFP1+ and RFP1- cells were isolated by cell sorting and the endogenous levels of pSmad2 were quantified by Western blot. RFP+ had almost 5 times more pSmad2, compared to RFP- cells, as expected. |
| Breeding history | Bred by crossing to C57BL/6 background. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | heterozygous C57BL/6 males, wild-type C57BL/6 females |
| Stage of embryos | Morula |
Literature references
- Coordinated activation of TGF-β and BMP pathways promotes autophagy and limits liver injury after acetaminophen intoxication.;Stavropoulos Athanasios, Divolis Georgios, Manioudaki Maria, Gavriil Ariana, Kloukina Ismini, Perrea Despina N, Sountoulidis Alexandros, Ford Ethan, Doulou Athanasia, Apostolidou Anastasia, Katsantoni Elena, Ritvos Olli, Germanidis Georgios, Xilouri Maria, Sideras Paschalis, ;2022;Science signaling;15;eabn4395; 35763560