STOCK Tg(CAG-Arl13b/GFP)/Orl
| Status | Available to order |
| EMMA ID | EM:07263 |
| Citation information | RRID:IMSR_EM:07263 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Tg(CAG-Arl13b/GFP)/Orl |
| Alternative name | pCAGGS-Arl13bGFP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CAG-Arl13b/GFP) |
| Gene/Transgene symbol | Tg(CAG-Arl13b/GFP) |
Information from provider
| Provider | Nathalie Spassky |
| Provider affiliation | Institut de Biologie de l'Ecole Normale Supérieure, INSERMU1024, CNRS UMR 8197 |
| Genetic information | The GFP reporter is fused to Arl13b cDNA and put under the control of pCAGGS promoter expression. Four different founder lines (each obtained from a different pronuclear injection) were generated and all were confirmed to specifically express GFP in cilia, while no GFP could be seen in other cell organelles. Only the one line that expresses the highest level of GFP was maintained. The pCAGGS promoter was chosen because it can drive the strong and ubiquitous expression of the Arl13b-GFP fusion protein. The goal was to get primary cilia expressing GFP without any GFP in other parts of the cells. The Arl13b-GFP fusion protein has already been used to label cilia in vitro (see Larkins et al., Mol Biol Cell. 2011 Dec;22(23):4694-703). |
| Phenotypic information | no apparent phenotype |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNRS-TAAM – Typing and Archiving of Animal Models, Orléans, France |